How do plants respond to drought

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How do plants respond to drought?
Sandra Porter Digital World Biology Sandra_at_digital
worldbiology.com
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The experiment
Arabidopsis, several plants Sample duplicate
samples over time (0.25, 0.5, 1, 3, 6, 12, 24hrs)
Isolate RNA - hybridized to microarray chips
(22,000) Deposited data in GEO - a database at
the NCBI
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Measuring gene expression
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Analyze data

• Pre-process data during upload step
• Used to normalize data within chips and between
  chips
• Use statistics to help identify differences in
  expression between samples and correct for
  multiple testing
• T test, and Benjamini and Hochberg, set levels
• Explore the biology