Transfer Disinfection Validation

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Transfer Disinfection Validation

• Sarah Hiom
• Cardiff and Vale NHS Trust

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Aseptic PreparationIsolator Technology
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Transfer into controlled work zone
? High contamination risk ? Disinfection (70
alcohol)
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Validation of Disinfection Transfer

• Current methods
• Swabs/Contact plates
• Lack sensitivity
• Not appropriate for assessing disinfection

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Aim

• To develop and validate an accurate in house
  disinfection assessment method
• To use this method to benchmark contamination
  levels and compare disinfection transfer
  procedures in pilot sites across Wales

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Method
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Validate new method

• Background count
• Effect of residual alcohol on recovery rates
• Determining recovery

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Recovery

• S.epidermidis stock culture 55cfu/100µl

Contact Plate 40 Swab 20
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Sampling Concept
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Benchmark Contamination Levels

• 50ml glass vial
• Batch (6) stored in unit for 5 days
• Bioburden determined after each disinfection
  point during transfer to critical work zone (n ?
  5)

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Pilot conclusions

• Novel sensitive method to assess disinfection
  transfers
• Spray wipe transfer methods significantly
  better at reducing bioburden than spray only
  procedures (Plt0.1)
• Better bioburden reduction when vial caps opened
  before alcohol spray disinfection transfers
  (Plt0.1)
• Operational procedures showed transfer of up to 9
  cfu/vial into critical work zones

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• Action limits?

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Bioburden (cfu/item)
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Action Limits

• for transfer disinfection procedures to pass
  this validation test, results are expected to
  demonstrate no greater than two colony forming
  units from each item sampled within the critical
  work zone.

SteriVal Technical Information Sheet 3.3