Ab Binding Sites, Polyclonal and Monoclonal Antibodies

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Ab Binding Sites, Polyclonal and Monoclonal
Antibodies

• Chris Chase
• September 29, 2004

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Lecture Outline

• Effector mechanisms
• Passive antibody protection
• Immunoglobulins function structure
• Monoclonal antibodies

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Humoral effector mechanisms NEUTRALIZATION
NEUTRALIZING ANTIBODIES
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Antibody Effector Mechanisms
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Humoral effector mechanisms against extracellular
pathogens OPSONISATION
binding
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Humoral effector mechanisms against extracellular
pathogens COMPLEMENT
Lysis
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Passive Antibody Immunity

• Maternal Passive Antibody
• Humans and mice- Moms antibody is transferred to
  the fetus in last 3 months of pregnancy
• Cattle, horses, pigs and sheep- Maternal antibody
  is concentrated in the milk called colostrum.
  Transfer occurs in the 1st 12-24 hrs of life.

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Passive Antibody Immunity

• Therapeutic Passive Antibody
• Antibodies against pathogen developed in the same
  species or different species
• Example, rabies antiserum
• From different species, eventually get serum
  sickness

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Making Human Polyclonal Antibody in Cows
INSERT HUMAN IMMUNOGLOBULIN GENES
MICROCHROMOSOME
HEAVY CHAIN GENE
LIGHT CHAIN GENE
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Making Human Polyclonal Antibody in Cows
MAKE COW FROM GENETICALLY MODIFIED CELL
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Making Human Polyclonal Antibody in Cows
IMMUNIZE COW WITH HUMAN DISEASE VACCINE
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Making Human Polyclonal Antibody in Cows
COLLECT BLOOD AND ISOLATE HUMAN ANTIBODIES
Rx
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Antibody Classes and Biological Activities

• IgG monomer 80 of serum antibody
  IgG1gtIgG2gtIgG3gtIgG4
• IgM pentamer 5-10 of serum antibody
• IgA monomer-serum, dimer-mucosal surfaces 10-15
  of serum antibody
• IgE monomer least amount lt0.001
• IgD monomer 0.2 of serum antibody

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B cell Receptor
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The Immunoglobulin Superfamily
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Monoclonal and Polyclonal Antibodies
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Therapeutic Uses of Antibodies against Tumor
Cells
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