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Coop Takeshi, Xiao-Ru, Dag, Anders

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Pollen contamination and self-fertilization, within V sterhus in 9 loci ... of each clone as a pollen donor when assuming null alleles in 9 loci ... – PowerPoint PPT presentation

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Title: Coop Takeshi, Xiao-Ru, Dag, Anders


1
Västerhus information and some results Last
edit 080808
2
Selection of clones from six progeny-tests (old
orchards)
Seed orchard Seed orchard Tested Available Normal order statistics of ith ranking ( BV) Normal order statistics of ith ranking ( BV) Normal order statistics of ith ranking ( BV) Normal order statistics of ith ranking ( BV) Normal order statistics of ith ranking ( BV)    
No Name clones clones 1 2 3 4 5 6 7
412 Domsjöänget 52 42 2.180 1.776 1.542 1.370 1.232 1.114 1.009
        AC1006 Y4507 AC2064 Z3009 AC3040 AC3033 AC4221
410 Robertsfors 12 10 1.539 1.001 0.656        
        Y3014 AC3065          
18 Brån 34 27 1.998 1.563 1.306 1.115 0.957 0.820 0.699
        AC3056 Y3001 AC1075 AC2047      
403 Nedansjö 40 32 2.070 1.647 1.400 1.217 1.067 0.938 0.824
        Y4016 Z2081 Z4003 X4203 Z4019    
411 Domsjöänget 50 40 2.161 1.753 1.517 1.344 1.203 1.083 0.977
        Y2005 Y3012 Y2004 Z4022 Z3029 Z4032 AC3015
426 Holm 20 16 1.766 1.285 0.990 0.763 0.570    
        Z3007 Y4508 Y4103        
3
Clone ID Breeding Value Percentage Clone ID Breeding Value Percentage
AC1006 2.2 7 Z4022 1.3 3
Y2005 2.2 8 AC1075 1.3 2
Y4016 2.1 7 Y4508 1.3 3
AC3056 2.0 9 AC3040 1.2 3
Y4507 1.8 6 X4203 1.2 2
Z3007 1.8 6 Z3029 1.2 2
Y3012 1.8 6 AC2047 1.1 1
Z2081 1.6 6 AC3033 1.1 1
Y3001 1.6 4 Z4032 1.1 1
AC2064 1.5 5 Z4019 1.1 2
Y3014 1.5 5 AC4221 1.0 2
Y2004 1.5 2 AC3065 1.0 1
Z4003 1.4 3 Y4103 1.0 1
Z3009 1.4 2 AC3015 1.0 1
4
                                                                                                                    
Recommended area for using the seed orchard Västerhus (T10) Latitude for plus tree origin 6330' Category Tested Location Latitude 63,8 N Elevation 15 m Established 1991
     
5
Range of origins v 3 latitudes, but
considering pollen, range of halfsibfamilies v/2
1.5 latitude
Plust tree origins
Seed orchard location
6
Relative performance over plantation sites
compared to an optimal seed orchard (100)
7
Map. Västerhus missing Z3007 clone ID
Parcells (plots) and in plots X for column and Y
for rowThis is actually the only way to find the
identity of a particular clone in the
orchard! Z3007-82-1-3
8
Västerhus (Anders F 07-10-10)
9
Air picture of Västerhus
10
Västerhus spring 2006
Västerhus spring 2006. Czech visitors. Photo
Morogushi
11
(No Transcript)
12
Anders and me selected study trees and harvested
needles
  • Criteria 3 ramets per clone
  • Possible to study pollen spreading from rare
    clones
  • Somewhat spread over the orchard
  • Needles in Xiao-Rus lab

13
Cone collection
14
Simple Breeding without Breeding Cycle
Forest
Group merit selection
Seed orchard
15
Västerhus
  • We are anyway genotyping the seed orchard and its
    offspring
  • There are a few mature (30 year) crosses among
    seed orchard trees (in old seed orchards,
    Domsjöänget). Johan Kroon will help us to find
    some such trees and we will check the
    possibilities to make phenotypic selection
    followed by parent determination in a situation
    where we know the parents. Not many trees. A few
    days in the summer 2008 to look at the material.

16
Mature seed orchard Domsjöänget, 25 kms from
Västerhus. These trees were crossed in the
orchard and the progeny planted in field trial.
The field trials were evaluated and the best
clones placed in Västerhus. Some of the crosses
are between Västerhus clones and exist in a 30
year old progeny-test where we collected needles
June 2008. When we can evaluate the chance that
we can identify the parents
17
  • Trees at the progeny trial Kvarntorp with crosses
    at Domsjöänget at needle collection June 2008
  • Needles are collected by cutting branches down
    with a pole with a saw (at the centre of the
    picture)

Photo Takeshi Torimaru 0806
18
Plantation with Västerhus seeds
19
Plantation with Västerhus seeds
20
Harvesting needles from plantation with Västerhus
21
Half sib forestry
  • I and Anders have applied to Kempe in coop with
    the chief silviculturist at Holmen and the seed
    manager of SSP (Lagan, Finnvid). We did not get
    money.
  • We applied in Bo Rydin but they suggested no
    spring meeting
  • We applied föreningen but they did not want to
    initiate something without the full board.
  • We now have an application at Bo Rydin and will
    send one to föreningen
  • Thus we wait till 2009 with the seeds. One
    advtage is that the pollen production dropped
    2007, but hopefully will raise again 2008.
  • Harvesting 25 of area of Västerhus clonewise
  • The number of seeds will be counted for each
    clone, thus the female fertility. This can be
    combined with our measurements of male fertility
  • Follow these harvests through seeds, nursery to
    the field.
  • Ten halfsib families are chosen for trials
  • 10 field plantations around 20 hectars each with
    the 10 lots with half sibs and 3 (as comparison)
    with mixtures
  • We may revise BWB to be applied to half sib
    forests, when we know the mother

22
Pollen contamination and self-fertilization,
within Västerhus in 9 loci
Null alleles assumed to be Null alleles assumed to be Null alleles assumed to be Null alleles assumed to be Null alleles assumed to be Null alleles assumed to be
partly present partly present
Contamination () 51,63 ( 3,95)
Selfing () 1,30 ( 0,62)
Outcrossing within seed orchad () 47,07 ( 4,05)
Standard error in parentheses
Contamination is high like in most similar
studies!! Remains a very big and unsolved problem
Selfing is low compared to similar earlier
studies, non problematic level
23
Expected (black bars) and observed (grey bars)
contributions of each clone as a pollen donor
when assuming null alleles in 9 loci
Number of seeds
Observed
Expected
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