Facility for Genomics and Proteomics and UFSNBN Bioinformatics Node

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Facility for Genomics and Proteomics and UFSNBN Bioinformatics Node

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Facility for Genomics and Proteomics and UFSNBN Bioinformatics Node –

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Title: Facility for Genomics and Proteomics and UFSNBN Bioinformatics Node


1
Facility for Genomics and ProteomicsandUFS-NBN
Bioinformatics Node
2
Facility for Genomics and Proteomics
X
Lab 39A Biotechnology Building
3
Equipment available
  • AKTA FPLC
  • Purification of proteins
  • Characterization
  • Crystallization
  • Identification
  • LC Methodologies
  • Affinity
  • Size exclusion
  • Ion-exchange
  • Unicorn control software

4
GenePix Pro Microarray slide scanner
  • Scanner for DNA microarray slides
  • GenePix Pro software
  • 2 Channel 532nm and 635nm laser
  • Cy3/Cy5 dual channel scans
  • Transcriptomics
  • Acuity software

5
Pharos Plus 2D gel scanner
  • 2D scanner
  • 3 lasers 488nm, 532nm and 635nm
  • Quantity One software
  • PD Quest software

6
2D protein gels
Cy3/Cy5 DIGE (fluorescence)
35S SILAC (phosphorimage)
7
Pharos Plus Scanning Options
8
4000 QTRAP Hybrid Triple Quadrupole Mass
Spectrometer
  • Nanoflow HPLC (1?l.min-1)
  • ESI LC-MS-MS
  • Proteomic application
  • Peptide mass fingerprinting
  • Search database library
  • De novo peptide sequencing
  • Post-translational modification analysis
  • Cross-linking analysis (MS3D)
  • MIDAS

9
UFS-NBN Bioinformatics Node
X
10
Bioinformatics Lecture Hall
  • 26 Quadcore PCs
  • Dual boot
  • Windows XP
  • Fedora Linux
  • R (stats and graphs)
  • Python
  • Bioinformatics teaching
  • Specialized workshops

11
Dedicated Bioinformatics server
  • Dual quadcore Xeon CPUs
  • 1 Terra Byte RAID
  • Linux 7 Enterprise
  • Apache
  • MySQL
  • Python
  • Dedicated bioinformatics databases
  • Analysis packages
  • Local Programs
  • SSH Access from off-campus

12
Contact
  • Hugh Patterton
  • X2274
  • patterh.sci_at_ufs.ac.za
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