Presented by Qiong Cheng in cs6640

1
Identify an Obesity Association INSIG2

• Presented by Qiong Cheng in cs6640
• Instructor Prof. Irene T. Weber
• Prof. Robert W. Harrison

2
Outline

• Introduction
• Analysis Methods
• Analysis Results
• Conclusion

3
Introduction

• Obesity
• - an excess of body fat
• - a known risk factor for chronic diseases
• - a kind of metabolic disease
• INSIG Family and INSIG2

4

• INSIG Familys Role

5

• INSIG2 is an attractive candidate protein which
  product inhibits the synthesis of fatty acid and
  cholesterol 1.

6
Analysis Methods

• NCBI gene/protein database searching
• Blast Alignment searching in PDB database
• PDB database searching
• LALIGN for pairwise alignment
• ClustalW for multiple alignment
• Newho preammp AMMP structure alignment tools
• Rasmol to show protein structure

7
Analysis Results NCBI-Result

• INSIG2 sequence
• 1 maegetespg pkkcgpyiss vtsqsvnlmi rgvvlffigv
  flalvlnllq iqrnvtlfpp
• 61 dviasifssa wwvppccgta savigllypc idrhlgephk
  fkrewssvmr cvavfvginh
• 121 asakvdfdnn iqlsltlaal siglwwtfdr srsgfglgvg
  iaflatvvtq llvyngvyqy
• 181 tspdflyvrs wlpciffagg itmgnigrql amyeckviae
  kshqe
• //

8
Analysis Results BLAST-Result
9

• Alignment of INSIG2 and 1R08 in LALIGN

• Alignment of INSIG2 and 1RHI in LALIGN

10

• Alignment of INSIG2, 1R08 and 1RHI in ClustalW

• Alignment Tree Generated in ClustalW

11
Analysis Results AMMP-Predicted INSIG2

• Molecule Display--- Ribbons Display

12
Analysis Results AMMP-Predicted INSIG2

• Molecule Display--- Ribbons Display

13
Analysis Results Comparison-1 in RasMol

• Predicted INSIG2 --- 1R08 in 3rd chain

14
Analysis Results Comparison-2 in RasMol

• The predicted INSIG2 is the red one
• 1R08 in 3rd Chain is the green one

15
Analysis Results Comparison-2 in RasMol

• The predicted INSIG2 is the red one
• 1R08 in 3rd Chain is the green one

16
Analysis Results The longest gap

• Seek motif SAK
• Seek the first residue after the largest gap
• Identify the gap
• in RasMol Tool

17
Conclusion

• Study research topics and tools in
  bioinformatics
• Identify INSIG2 structure
• The interesting thing is macromolecule structure
  homology
• Further experiments and identification are
  needed
• References
• Alan Herbert, Norman P.Gerry A Common Genetic
  Variant is Associated with Adult and Childhood
  Obesity Science, Vol312 (2006).

18
Analysis Results Sequence Alignment

• Alignment of INSIG2 and 3rd chain of 1R08
  (HRV14)