Nanbiosensor Working Group, Nov. 19th, 2002

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Nanbiosensor Working Group, Nov. 19th, 2002
Convener Antje Baeumner Participants Evangelyn
Alocilja Larry Branen Paul
Dawson Richard Durst Joseph
Irudayari Zhiko Kounev Greg
McCarty Jerzy Nowak Daniel
Schmold Ruixiu Sui U. Sunday Tim John
Vetelino Guigen Zhang
www.nseafs.cornell.edu
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Background and Rational of Nanobiosensors

• Bioanalytical Nanosensor
• using biology or used for biology
• Collection, interaction, sensor platform,
  response
• - Biosensor system, portable and non-portable

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Background and Rational of Nanobiosensors

• Characteristics of Biosensors
• - single use remote, small, portable, rapid
  response (real time) and processing,
• specific, quantitative
• Laboratory more specific (i.e. subtyping
  capability)
• non-portable, high throughput, quantitative

• Multichannel
• Sensitive
• Reliable
• Accurate
• Reproducible
• Robustness
• Stability

• 6 more slides on background and rational

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Background and Rational of Nanobiosensors
Pathogen Detection Foods, Plants, Animals,
Fruits Vegetables Plant/Animal Production,
Transgenics Cloning BioProcessing, Foods ,
Industrial Products (BioProcessing, Food Quality
Safety) BioSensor, (Biosecurity)
Agrosecurity Environmental Processing
(Environmental Monitoring) Sustainable
Agriculture (Sustainable Rural Community)

• 6 more slides on background and rational

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Specific Objectives in Bioanalytical Nanosensors
1. Novel nanomaterials adaptable to agricultural
food systems (limited sample preparation,
complex systems (dirty), more robust)
2. Novel recognition mechanisms based on the
nanoscale (fundamental studies,
non-biomaterials, biomimetics, carbon nanotubes,
MIP, recombinant genetically engineered)
3. Novel integration mechanisms of transducer and
recognition element (SAM, Directed/guided
assembly, new immobilization (chemical,
biological and electrical)
4. Novel transduction mechanisms (mechanical,
impedance, piezoelectric, optical,
electrochemistry, transistor)
5. Integration of NEMS remote receive/transmit
systems (embedded on the chip, satellite
interaction, GPS, remote powering biopowering)
Education about Nanotechnology as overall
educational topic area for initiative
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Potential Outcomes Impacts in Nanobiosensors
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Crosscutting Issues

• Collection Transport, Bioseparation,
  Microfluidics, Bioselective surface
• Sampling representative sample (statistically
  significant)

• Recognition Bioselective Surface, Microfluidics,
  Nanomaterials, Nucleic Acids
• specificity (saturation,time, fouling)

• Transduction Bioselective Surface, Microfluidic,
  Nanomaterial
• Integration to bioselective surface

• Data Processing Nanomaterials

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Crosscutting Issues (continued)
Drug delivery Sensing if release is required,
where to release NanoBioprocessing Modeling
Models for new nanosensors nanosensors
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Estimated Research Budget for Bioanalytical
Nanosensors
Fundamental Theme area Centers of
Research Education Total research
challenge excellence
infrastructure in million (applied)
(equipment)
1 1 1 2 1 1 3 1 1 4 1 1 5 0.4
0.4 3 projects per year at
300,000 2 projects per year at
200,000 8.8
1 1 1 4 regional 2 1 1 centers 3 1 1
(5 year) 4 1 1 10/year 5 0.4 0.4
3 projects per year at 300,000 2
projects per year at 200,000 18.8
1 2 3 4 5

• 1
• 1
• 1
• 1
• 5 0.4
• 3 projects per year at 300,000
• 2 projects per year at 200,000 4.4

1 1 1 4 regional 1 2 1 1 centers 1
3 1 1 (5 year) 1 4 1 1 10/year 1
5 0.4 0.4 3 projects per year
at 300,000 2 projects per year at
200,000 22.8
1 1 1 4 regional 1 0.2 2 1 1 centers 1
0.2 3 1 1 (5 year) 1 0.2 4 1 1 10/y
ear 1 0.2 5 0.4 0.4 0.2 3
projects per year at 300,000 2 projects per
year at 200,000 23.8
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• Biosensors Definition of Biosensor Systems
• Bioanalytical Nanosensor
• using biology or used for biology
• Collection, interaction, sensor platform,
  response
• - Biosensor system, portable and non-portable
• Characteristics of Biosensors
• remote, small, portable, rapid response (real
  time) and processing, single use,
• Specific, quantitative
• Laboratory base more specific (I.e. subtyping
  capability) non-portable, high throughput
• quantitative
• Multichannel
• Sensitive
• Reliable
• Accurate
• Reproducibility
• Robustness

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• Rapid sensor (min or less)
• Simple
• In-situ (real-time)
• Detection at farm-level, and throughout food
  processing and distribution chain,
• including consumer
• Non-invasive (non-destructive)
• Remote
• Telemetric
• we include FOOD SAFETY
• HACCP
• Examples
• implanted sensors in farm animals
• Monitoring of pathogens throughout the farm for
  early recognition of disease
• Tracing sources of contamination (typically
  done in the lab)
• Sample egg wash water, telemetric detection and
  automatic adjustment of disinfectant
• Fish farms
• Mycotoxin levels in grains
• Toxins from pathogenic organism

Pathogen Detection Foods, Plants, Animals Fruits
Vegetables Plant/Animal Production Transgenics
Cloning BioProcessing Foods Industrial
Products BioSensor (Biosecurity)
Agrosecurity Environmental Processing Sustainab
le Agriculture
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Pathogen Detection Foods, Plants, Animals Fruits
Vegetables Plant/Animal Production Transgenics
Cloning BioProcessing Foods Industrial
Products BioSensor (Biosecurity)
Agrosecurity Environmental Processing Sustainab
le Agriculture

• - GMO detection throughout processing/distribution
  including consumer
• Detection the function of the immune system in
  animals to detect disease resistance
• (production efficiency vs. disease)
• rapid screening of clones
• SMART plants plants sensor
• Production process
• increase yield, productivity by monitoring the
  animals and plants
• Root/soil interface investigation
• (ethylene ripening)
• IPM
• Ask experts in this field
• Specifics demanded of biosensors
• GMO --- rapid, simple etc.
• others fast, but in the lab is sufficient

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• BioProcessing, Food Quality Safety
• Monitoring of Food Quality Safety
• Contamination, physical properties
• - heavy metals, pesticides
• - oxidation
• - metabilites, amonia etc.
• - antioxidants
• - texture
• On-line monitoring of bioprocessing (proteins,
  cheese)
• Monitoring imported foods
• Characteristics
• - both, portable and lab

Pathogen Detection Foods, Plants, Animals Fruits
Vegetables Plant/Animal Production Transgenics
Cloning BioProcessing Foods Industrial
Products BioSensor (Biosecurity)
Agrosecurity Environmental Processing Sustainab
le Agriculture
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Pathogen Detection Foods, Plants, Animals Fruits
Vegetables Plant/Animal Production Transgenics
Cloning BioProcessing Foods Industrial
Products BioSensor (Biosecurity)
Agrosecurity Environmental Processing Sustainab
le Agriculture

• Take everything from Pathogen Detection

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Pathogen Detection Foods, Plants, Animals Fruits
Vegetables Plant/Animal Production Transgenics
Cloning BioProcessing Foods Industrial
Products BioSensor (Biosecurity)
Agrosecurity Environmental Processing Sustainab
le Agriculture

• Environmental Monitoring, (quality)
• Water
• Surface,
• ground,
• watershed
• Waste water
• Air
• Odor
• Spores,
• particulates
• allergens
• Soil
• Nutrient monitoring in soils (phosphorus, carbon,
  nitrogen)
• Characteristics of biosensors needed

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Pathogen Detection Foods, Plants, Animals Fruits
Vegetables Plant/Animal Production Transgenics
Cloning BioProcessing Foods Industrial
Products BioSensor (Biosecurity)
Agrosecurity Environmental Processing Sustainab
le Agriculture

• Sustainable Rural Community
• Environmental Monitoring, (quality)
• Production
• BioProcessing
• ALL OF them fit into this category
• Having nanosensors helping in making decisions,
  not only for monitoring
• Even opens up opportunities between rural urban
  discussions
• Agriculture in the classroom
• Nanosensors as example of teaching tool, I.e.
  high tech to Agriculture, to K-12