Stephen Fish, Ph'D'

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Stephen Fish, Ph.D. Marshall University J. C. E.
School of Medicine Fish_at_Marshall.edu
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Note to instructors I use these PowerPoint
slides in cell biology lectures that I give to
first year medical students. Copy the slides, or
just the illustrations into your own teaching
media. We all know that teaching science often
requires compromises and simplification for
specific student populations, or the requirements
of a specific course. Please feel free to offer
suggestions for improvements, corrections, or
additional illustrations. I would be pleased to
hear from anyone who finds my work useful, and am
always willing to make it better. Also, the
images have been compressed to screen resolution
to keep PowerPoint file size down, and I can
provide them at any resolution. Stephen E.
Fish, Ph.D.
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Golgi Traffic
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Golgi apparatus
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Golgi apparatus
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Golgi trafficdiagram
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Reversetraffic

• BiP escapes ER
• Binds return receptor in low pH
• Released in neutral pH

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Two versions of how Golgi transport works-
probably both
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Golgi N-linked (NH2 on asparagine) glycosylation
Complex oligosaccharides
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Golgi N-linked glycosylation High mannose
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O-linked glycoproteins
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Loading lysosomes
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Two types of secretion
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Make a secretory vesicle (Click through sequence)
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Pancreatic acinar cell

• Colicystokinin triggers zymogen secretion
• 30 X cell area added by vesicles in minutes
• Must be same area removed by endocytosis

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Sherman says
Lets do something else