Osler Institute Pathology Board Review: Electron Microscopy

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Osler Institute Pathology Board Review
Electron Microscopy
EM of Tumors and Tumor-Like Entities
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Osler Institute Pathology Board Review
Electron Microscopy

• Acinar Cell Carcinoma
• Zymogen granules 0.3 1.5 microns
• Stacks of RER
• Salivary gland
• Pancreas
• Pancreaticoblastoma
• Immunopositive for
• alpha-1 antitrypsin
• Trypsin
• Chymotrypsin
• Lipase

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Osler Institute Pathology Board Review
Electron Microscopy
Adenocarcinoma extracellular lumina, intestinal
type microvilli
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Osler Institute Pathology Board Review
Electron Microscopy

• Neoplasms
• Adenocarcinoma
• Intracytoplasmic lumina
• Microvilli
• Junctional complexes

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Osler Institute Pathology Board Review
Electron Microscopy
Neoplasms
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• Neoplasms
• Bronchioloalveolar Cell carcinoma
• Type II Pneumocyte
• Myelin figures
• Microvilli
• Clara Cells
• Secretory granules

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• Neoplasms
• Carcinoid (Neuroendocrine) Tumors
• Dense core granules
• 140 400 nm
• True lumens
• Intracytoplasmic filaments

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• Eosinophilic Granuloma
• Langerhans cell histiocyte
• Rod shaped Birbeck Granule
• Pleomorphic, deeply cleaved nucleus

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• Neoplasms
• Ependymoma
• 5 7 of intracranial neuroglial neoplasms
• 60 of neuroglial tumors in spinal cord
• Most common sites fourth ventricle and
  lumbosacral region
• Flexner type rosettes
• True lumens cilia, microvilli and junctional
  complexes

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• Neoplasms
• Ependymoma
• Junctional complexes
• Intracytoplasmic lumina
• Intracytoplasmic glial filaments
• Immunopositive for GFAP

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• Neoplasms
• Granular Cell Tumor
• Pleomorphic secondary lysosomes
• Basal lamina around cell groups

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• Neoplasms
• Granular Cell Tumor
• Angulate lysosomes in stromal fibrohistiocytic
  cells

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Neoplasms

• Hibernoma
• Lipid droplets
• Electron dense mitochondria
• S-100 protein
• Vimentin

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• Lymphoma
• Non Hodgkin's Type
• No junctional complexes
• Few cytoplasmic organelles
• Abundant polyribosomes

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• Lymphoma
• Non Hodgkin's Type
• No junctional complexes
• Few cytoplasmic organelles
• Abundant polyribosomes

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• Neoplasms
• Mesotheliomas
• Long thin microvilli
• Bipolar cytoplasmic processes
• Desmosomes
• Primitive junctions
• Calretinin
• Mesothelin