Aseptic technique

1
Aseptic technique
2
Before you start, get a bottle of O-SYL and
wipe down your bench with it
3
You will need a flame to light the bunsen burner

• If you are using a torch, do NOT keep striking
  it, if there is no spark your fellow students
  would like to continue to breathe
• You will NEVER get a bunsen burner to light if
  there is no spark in the torch

4
Turn on the blue knob to provide gas and to light
the bunsen burner
5
Your flame does not need to be very high adjust
it, if necessary
6
It is now time to start working with your
bacterial culture
7
Your right pinky is now the most important part
to perform the next steps

• Hold the tube with the bacterial culture with
  your left hand
• Remove the cap with your pinky from your right
  hand
• In the aseptic technique, you want to try NOT to
  put anything down on the counter if you do not
  have to

8
Every time you open or close your bacterial
culture, you want to flame your tube

• Remember, the cap is being held by your pinky of
  your right hand and is NOT on the counter
• With the tube in your left hand, pass it through
  the flame
• If you need to use the loop, hold it between your
  thumb and forefinger of your right hand

9
Close the tube by transferring the cap from your
right hand onto the cover of the tube
10
Time to clean up

• When you are done with the cultures, place them
  in the rack or container provided by your
  instructor
• Make sure that you have turned off the flame
• All bacterial plates that you do not want must be
  placed in the orange bags
• Tubes that you do not want should be placed in
  the RACK in the back of the room they are not
  discarded
• Wipe down your bench with O-SYL