MCB 141

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MCB 141 Developmental Biology
Lecture 5 Feb. 3, 2009
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Temporal aspects Gurken signals during egg
development within the female ovary Dorsal does
not move into nucleus until the embryo has
reached cycle 10 (two hrs after fertilization and
laying) Rescue of eggs laid by Gd/Gd can
still be carried out after egg laying
(injection of Gd protein)
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XXX ventralized
What is wrong here?
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XXX ventralized
This part is fine
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XXX ventralized
What is wrong here?
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XXX ventralized
This is correct, but only if the torpedo
deficient mother is mated to a torpedo deficient
father for donor pole cells. Need cells to be
torpedo/torpedo AND Recipient embryo was
produced by torpedo/torpedo female mated to a
male that was tor/tor or tor/tor
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XXX ventralized
Make sure you understand what happens if you do
the same analysis with gurken mutation. Your
book says that gurken mRNA is made by nurse
cells (not just oocyte nucleus). Why would this
not change the outcome of this transplantation
experiment?
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Also found maternal effect mutants Three
classes terminal, anterior missing, posterior
missing Two key ones - bicoid (anterior
missing) and nanos (posterior) missing
Embryos from bcd-/bcd- mothers
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nanos mRNA
bicoid mRNA
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Microtubules are polarized (/- ends) end is
posterior Proteins like swallow are motors that
carry cargo, like bcd mRNA) Some motors go in
direction, others go in - direction.
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bicoid protein
bicoid mRNA
bicoid protein
bicoid protein
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nanos
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Gap mutants large contiguous deletions Krüppel g
iant knirps hunchback
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Pair-rule Two segment periodicity fushi
tarazu even-skipped paired odd-skipped runt hairy
odd-paired sloppy paired
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So what if we instead used
GFP
brain enhancer
digit enhancer
spine enhancer
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So what if we instead used
GFP
digit enhancer
spine enhancer
or
GFP
brain enhancer
spine enhancer
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X
X
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Segment polarity Deletions/duplications
in every segment gooseberry engrailed hedgehog w
ingless patched armadillo etc.....
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Gene X mRNA
Gene X protein
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B F
C G
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Posterior cool Anterior warm 65-100 min
Anterior warm Posterior cool 100 - 180 min
Anterior warm Posterior cool 0-65 min