LSRII default 488

1
LSRII default 488
BLUE 488nm
PerCP, PerCP-Cy5.5, PE-Cy5
Default LSR II Configuration
FITC Alexa488
695/40
685 LP
530/30
505LP
488/10
550 LP
SSC
735 LP
576/26
780/60
PE
PE-Cy7
2
LSRII default 633, 407
Default LSR II Configuration
For A700 we need 710/50, LP685 in B
RED 633nm
VIOLET 407nm
APC
PacificBlue, Alexa405
660/20
440/40
735 LP
505 LP
780/60
525/50
AmCyan, Alexa430, Cascade Yellow, CFP
APC-Cy7, A750
3
LSRII default 355
Default LSR II Configuration
Hoechst (blue) DAPI
UV 355nm
440/40
505 LP
530/30
4
LSRII YFP/GFP
PerCP, PerCP-Cy5.5, PE-Cy5
GFP/YFP LSR II Configuration
GFP
695/40
685 LP
510/20
505LP
488/10
SSC
525 LP
735 LP
550/30
780/60
YFP
PE-Cy7
5
LSRII calcium, SP
Stem Cell Side Population Dead Cell Exclusion
LSR II Configuration for Calcium Flux Assays
Hoechst (blue) DAPI
Indo-1 violet
UV 355nm
UV 355nm
440/40
405/20
505 LP
450 LP
585/42
530/30
Hoechst (red) PI (UV)
Indo-1 blue
6
Aria default 488
BLUE 488nm
Default FACSAria Configuration Use Aria 7-3-08
PerCP, PerCP-Cy5.5, PE-Cy5
PE
695/40
655 LP
576/26
556LP
530/30
502LP
FITC Alexa488
488/10
SSC
595 LP
755 LP
610/20
780/60
PE-TexasRed
PE-Cy7
7
Aria default 633, 407
Default FACSAria Configuration
PacificBlue, Alexa405 Hoechst Blue, DAPI
Alexa700 APC-Cy5.5
VIOLET 407nm
RED 633nm
720/20
440/40
680 LP
735 LP
502 LP
660/20
780/60
585/42
AmCyan, Alexa430, Cascade Yellow, CFP, Pacific
Orange
APC Alexa647
APC-Cy7
8
Aria GFP/YFP
PerCP, PerCP-Cy5.5, PE-Cy5
FACSAria GFP/YFP Configuration
YFP
695/40
685 LP
550/30
525 LP
510/20
505LP
GFP
600 LP
735 LP
610/20
780/60
PE-TexasRed
PE-Cy7
9
Aria SP
FACSAria Stem Cell Side Population Dead Cell
Exclusion
VIOLET
Hoechst Blue
440/40
505 LP
585/42
Hoechst Red PI (violet)
10
Dyes, Alternative Names, Filters Part I Antibody
Conjugates

• FITC 530/30
• PE 576/26
• PE-TexasRed (ECD, Red610) 610/20
• PerCP 695/40
• PerCP-Cy5.5 695/40
• PE-Cy5 (CyChome, PC5, TriColor, Red670) 695/40
• PE-Cy7 780/60
• APC 660/20
• APC-Cy5.5 720/20
• Alexa700 720/20
• APC-Cy7 (PharRed) 780/60
• AmCyan 530/30
• Alexa430 530/30
• PacificBlue 440/40
• Alexa405 440/40

11
Dyes, Alternative Names, Filters Part II DNA
Probes, Fluorescence Reporters, Functional Dyes

• GFP with YFP 510/20 (change mirror to 525LP)
  only GFP 530/30
• YFP with GFP 550/30 (change mirror to 525 LP)
  only YFP 576/26
• DsRed2 Aria 610/20 or 576/26 LSR II 576/26
  or 585/42 (change PE filter)
• CFP 530/30 (violet laser)
• Hoechst 33342 blue 440/40
• Hoechst 33343 red 585/42
• PI (blue laser) Aria 610/20 (PE-TexasRed) LSR
  II 695/40 (PerCP) CellCycle
• PI (Aria violet or LSR II UV) 585/42 Dead Cell
  Exclusion
• Indo-1 Calcium dye blue LSR II only 405/20
  (insert 450 LP mirror instead of 505 LP!)
• Indo-1 Calcium dye violet LSR II only 530/30
  (insert 450 LP mirror instead of 505 LP!)