Environmental Microbiology Lecture 7 Genetic exchange in bacteria - PowerPoint PPT Presentation

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Environmental Microbiology Lecture 7 Genetic exchange in bacteria

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Gene exchange (lateral gene transfer)? Mechanisms of genetic ... DNA is transferred by a bacteriophage. - Generalized transduction vs. Specialized transduction ... – PowerPoint PPT presentation

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Title: Environmental Microbiology Lecture 7 Genetic exchange in bacteria


1
Environmental Microbiology Lecture 7Genetic
exchange in bacteria
2
Why are bacteria so diverse?
Sexual recombination? Mutation? Gene exchange
(lateral gene transfer)?
3
Mechanisms of genetic exchange in bacteria
4
Transformation - free DNA is incorporated into a
recipient cell. - Griffiths experiments with
pneumococcus
5
Mechanisms of transformation
6
Transduction - DNA is transferred by a
bacteriophage. - Generalized transduction vs.
Specialized transduction
7
Conjugation - Genetic transfer by cell to cell
contact. - F plasmid
8
Genetic map of F plasmid
9
Integration of F plasmid into bacterial chormosome
10
(No Transcript)
11
Transposons and insertion sequences
12
Mechanisms of transposition
13
Integrons Transposons able to capture and
express genes from other sources
14
How to detect lateral gene transfer
  • Incongruence between phylogenetic tree
  • GC content
  • Patterns of codon usage
  • Insertion sequence determination

15
Scope of HGT in bacterial genome
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