HL60 differentiation to granulocytes with

1
- RA HL60 RA
HL60 differentiation to granulocytes with
all-trans-retinoic acid (ATRA)
2
HL60
-RA
RA
HL60 differentiation to neutrophils with
all-trans-retinoic acid (ATRA)
3
Granulocyte Differentiation
Neutrophil production 11 days Myeloblast Promye
locyte Myelocyte Metamyelocyte Band cell
10-12 µm dia Large spherical nucleus
lt15 µm Spherical nucleus Cytoplasm
basophilic Azurophilic granules
10-12 µm dia Nucleus ovoid, eccentric Granule
formation beginning
Nucleus has deep indentation Increased
chromatin density Granules in cytoplasm
Horseshoe-shaped nucleus, eccentric Increased
number in circulation usually means
infection Occasionally seen in peripheral blood