Lab

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Lab 15 FALL 2009Parasitic
WormsIntroductory Survey
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Experiments

• Helminth Parasites
• Vectors-Arthropods
• Streak Plate

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MICROBESHelminth Parasites

• Organisms for Study (Prepared Slides)
• Clonorchis (Chinese Liver Fluke) - adult
• Taenia (Tapeworm) - scolex and proglottid
• Enterobius (Pinworm) - adult
• Enterobius (Pinworm) - eggs
• Necator - (Human Hookworm) infective larvae
• Trichinella - (Encysted larvae)
• Use 4x - Do not use the High Power or Oil
  Immersion Objective or you may crack the slide
  (it is thick) or the objective.

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Helminths Parasites Phylum Platyhelminthes and
Phylum Nematoda

• Phylum Platyhelminthes (Flatworms)
• Flukes (Trematoda) and Tapeworms (Cestoda)
• Phylum Nematoda (Roundworms)
• Pinworm, hookworm, and ascaris

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Clonorchis fluke

• Phylum PlatyhelminthesClass TrematodaClonorchis
  sinensis - human liver fluke
• Chinese liver fluke
• Flat, bilaterally symmetrical, leaf like, pointed
  ends
• Parasites -EID
• Named for the region they infect
• Monoecious one house
• hermaphroditic

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Taenia Tapeworm

• Phylum Cestoda
• Adult scolex and proglottid
• Beef, Pork tapeworm
• taeniasis
• members of this genus have an "armed" scolex,
  meaning that there are hooks on the "head"
  region.
• Proglottids farthest from the head release eggs
  when deteriorating
• humans usually serve as a definitive host, eating
  infected meat, fostering adult tapeworms in the
  intestine, and passing eggs through feces,

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Enterobius Pinworm

• Phylum Nematoda
• Adult
• Eggs
• Small with long pointed tail
• Eggs
• have a characteristic shape, with an asymettrical
  form, flattened on one side
• Extremely contagious

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Necator Hookworm

• Phylum Nematoda
• Infective Larvae
• Live in the soil passed through the skin
• Cause anemia and malnutrition

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Trichinella Larvae (encysted)

• Phylum Nematoda
• Trichinellosis, also called trichinosis is caused
  by the roundworm Trichinella
• Ingestion of undercooked meat
• Cysts in skeletal muscle of infected animals
• excysted larvae then invade the intestinal
  mucosa, develop through four larval stages
• Symptoms include diarrhea, abdominal cramps,
  muscle pain, and fever.
• VIDEO

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Phylum Arthropoda Arthropods

• Tick- Class Arachnida
• Mosquito -Class Insecta
• Flea -Class Insecta
• Vectors-Transmit disease

West nile virus
The plague
Lyme disease
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TECHNIQUES UN Bacterial Unknowns Streak Plate
Method of Isolation

• Organisms (Broth cultures)
• MIX OF TWO UNKNOWN BACTERIA
• MARK YOUR NUMBER DOWN AND
• ALSO GIVE YOUR TO
• YOUR INSTRUCTOR
• Media
• Nutrient Agar plates
• Streak the plate using the pattern shown to you
  by your professor