The Alfin-like PHD Zinc Finger Transcription Factor Family

1
The Alfin-like PHD Zinc Finger Transcription
Factor Family

in Arabidopsis Thaliana
Melissa Borkenhagen8 May 2006 HC70AL DR. Bob
Goldberg
2
Gene AT5G20510

LBb1
FW
T-DNA
5 UTR
EXON
3 UTR
INTRON
T-DNA INSERT ACCORDING TO SALK
Chromosome 5
3
Gene AT3G42790

LBb1
T-DNA
FW
5 UTR
3 UTR
EXON
INTRON
T-DNA INSERT ACCORDING TO SALK
Chromosome 3
4
In which plant organs are the genes active?

Gene AT5G20510
Gene AT3G42790
Consistent with GeneChip Data, both genes appear
to be active in these plant organs.
5
Gene AT5G20510 Plant Genotypes

Separation of primers
Only one T-DNA Insert
Plant 1 is the only Homozygous Mutant Plant
Strange double bands What are the genotypes?
6
Gene AT3G42790 Plant Genotypes

Plants 1 and 4 are Homozygous Mutants
Plant 5 Heterozygous?
7
Are there any observable phenotypic differences
between wild type and mutant plants?

Wild Type
Mutant
No significant differences observed between wild
type and mutant plants.
8
Is continued work on this family of transcription
factors important?

• Research has shown that members of the alfin-like
  PHD zinc finger transcription factor family may
  be influential in plant development. Some appear
  to be particularly influential during seed
  development, with a possible role in the
  differential seedling development in darkness and
  light. Thus, continued work on these proteins is
  important!