PanSense: Rapid automatic platform for pointofcare testing

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PanSense Rapid automatic platform for
point-of-care testing

• TEKES IVD-meeting
• 15.6.2005
• Virve Hagrén
• University of Turku / Department of Biotechnology

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PROJECT

• PanSense and PanFlow separate projects, joint
  management group
• Project started in January 2005 ? July 2006

• Partners
• University of Turku
• Tampere University of Technology
• Abacus Diagnostica Oy
• Comsol Oy
• Finnzymes Oy
• Hidex Oy
• Innotrac Diagnostics Oy
• KCL Oy Keskuslaboratorio
• Perlos Oyj
• Plastone Oy
• Raisio Food Diagnostics Oy

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BACKGROUND

• Point-of-care testing (POC)
• vierianalytiikka lähitestaus
• human diagnostics
• environmental and food diagnostics
• Advantages of POC
• easy to perform
• quality
• cost-effective
• may save lives
• Currently, technological solutions are limited
• advantages of POC are lost, if some analytes have
  to measured in central laboratories

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AIM

• To develop a total technological solution for
  rapid point-of-care testing
• INSTRUMENT
• capable of performing various types of analytical
  measurements
• suitable for human, food or environmental
  diagnostics

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ASSAYS

• Nucleic acid assays
• for the measurement of viruses and cells in
  various sample matrices
• Immunoassays
• for the measurement of biomolecules in various
  sample matrices
• Clinical chemistry assays
• traditional, typically based on photometry

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• ? because all assay types can be performed with
  one instrument, POC can be implemented widely
• Technology is based on a prototype instrument and
  all-in-one dry chemistry assay concept
• time-resolved fluorometry
• rapid heating/cooling system
• reaction vessel enables various microfluidistic
  solutions

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• Principle of time-resolved fluorometry
• All-in-one dry chemistry assay concept
• all reagents are in dry form in the reaction
  vessel
• only the addition of a sample is required to
  start the assay

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OBJECTIVES

• Instrument related
• integration of the photometric measurement unit
• clinical chemistry assays
• microfluidistics
• PanFlow, pre-treatment of nucleic acid samples
• development of the reaction vessel
• PanFlow, material, one sample - many analytes,
  one analyte - many samples
• computer program
• analysis of the results, quality control

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• Assay related
• clinical chemistry assays
• photometry
• dry chemistry
• analytes calcium, glucose, alanine
  aminotransferase
• homogenous immunoassays
• SIMPLE no wash steps, just mix and measure
• nanoparticle labels, dry chemistry
• analytes estradiol and TSH

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• nucleic acid assays
• dry chemistry, RNA-analytes ? reverse
  transcription ? PCR
• analyte PSA

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PanSense group
Nucleic acid assays
Instrument related issues
Clinical chemistry assays
Immunoassays