Human Tissue Inc

1
Human Tissue Inc
Part I
Linda Kock Berdien Verboon Chia-Hui Yeh Marco
Janssen Joost Velterop
2
Skin - Structure

• Epidermis
• Keratin
• Dermis
• Fibroblasts
• Collagen
• Elastin
• Hypodermis
• Fat

3
Skin Temperature regulation

• Sweat glands
• Hairs
• Vascular constriction/dilation
• Shivering

4
Methods of skin replacement

• Autograft
• Allograft (Allogenic and Xenogenic)
• Synthetic replacement
• In vivo regeneration
• In vitro regeneration (ex vivo)

5
TE - Techniques

• Natural Matrix
• Synthetic Matrix
• Bilayer skin substitute
• Bioreactor?

6
TE - Properties

• Lack of dermal or epidermal layers (single layer
  grafts)
• Lack of hypodermis.
• Availability/ Growing time
• Absence of certain cells (e.g. melanocytes)
• Absence of sweat glands and hair follicles
• Lacking optimal fiber arrangement
• Lacking sensory functions

7
TE - Applications

• Skin grafts/ tissue grafts
• Victimless meat/ leather
• Bone constructions
• Self-adapting clothing/ furniture
• Flexible structures

8
Survey - Overall Results (1)
9
Survey - Overall Results (2)

• General Concerns
• Sources of Tissues
• Safety Issues
• Necessity
• Freedom of Choice
• Regulations and Responsibility

10
Survey Recommendations

• Clarify questions, for example with images
• Adapt goals to needs
• Better specified grades
• Ask for short motivations
• Subdivided open questions

11
Summary

• Technique is largely incomplete
• Size of TE products is insufficient
• People do not like controversial use of human TE
  tissue