Genetics Material

1
????????????
Genetics Material
DNA?
RNA?
Protein?
2
1928
Griffith
Diplococcus pneumoniae
??? colony ????????????????? capsule ???? ???
????????????? ??? colony ?????? ??????????
capsule ???? ???????????????????
Strain 3S
Strain 2R
3
Griffith
3S
Diplococcus pneumoniae
2R
3S
3S ?????
3S 2R
4
??????
?
Live 3S
Heat killed 3S
??? 3S ??? heat killed ???????????????????????
????????????? 3 ???????? 3S ??? heat killed ????
?????????? 3S ?????????? ??????????????
5
Griffith
2R
3S
Transforming factor
Transforming factor ?
6
Dawson Sia
1931
In vivo ?????????????????????????
In vitro ???????????????????????????
Heat killed
7
Avery, McCarty and McLeod
1944
3S Heat-killed
Protein DNA Polysacharide
8
DNA 3S heat-killed
Capsule DNA
Protein DNA
Protein Capsule
9
DNA 3S heat-killed
2R
3S
DNase
DNA 3S heat-killed
2R
2R
2R
3S
10
HersheyChase
Bacteriophage (Phage)
11
Life cycle of Bacteriophage
12
Protein - C, H, O, N, S
DNA - C, H, O, N, P
13
Phage ????????????????????????????
Phage ???????? ?????????????????????
14
DNA is Genetic material

• Griffith experiments
• Avery, McCarty and McLoad
• Hershey Chase

15
Tobacco Mosaic Virus
TMV
Fraenkel-Conrat
1952
16
(No Transcript)
17
???????? DNA RNA ?????? Genetic material ???
???????? ?????????? TMV ??? Fraenkel-Corrat
18
??????? ???????? 4
????????????????????? DNA ????
???????????????????? ????????????????????????????
??????????????????