TEM GRID vs. DIME

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TEM GRID vs. DIME
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How do they look under microscope?
                                                                                   
Square Grid
Hexagonal Grid

• 200 mesh
• 55 open area,
• 28 µm bar width
• Thick17.8 µm

3 mm
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TEM Grid Preparation

• There are two sides in carbon coated ultra-thin
  grid
• Formvar side - lighter side (less hydrophobic
  than the carbon film)
• Carbon film is on the darker side.

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TEM Grid Preparation

• Hold the grid with tweezers.
• Do not touch/contaminate the grid.
• Do not bend the grid.

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TEM GRID PREPARATION

• Take a micropipette and put a droplet on top the
  grid. Let the solution sit on the grid.
• After 10-12 min, wick away the rest of the
  solution with filter paper. Do not touch the
  grid!
• Finally let it sit until the grid dries off.