Murder - PowerPoint PPT Presentation

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Title:

Murder

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Restriction enzymes. DNA scissors. Cut at specific base pair sequence ... DNA looks different when cut with same restriction enzyme. DNA charge. Phosphate groups ... – PowerPoint PPT presentation

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Title: Murder


1
Murder?
  • Anyone know of a recent murder case from the news?

2
Dna Fingerprinting
  • Restriction enzymes
  • DNA scissors
  • Cut at specific base pair sequence
  • Lets us compare DNA from different sources
  • Diff. DNA looks different when cut with same
    restriction enzyme

3
DNA charge
  • Phosphate groups
  • Found between sugars of DNA structure
  • Negatively charged
  • Gives DNA an overall negative charge (polar)
  • Remember like charges repel
  • Opposites attract

4
Agarose
  • Sugar
  • Insoluble in water at room temperature
  • Soluble at higher temperatures
  • Melts and sugars connect to each other making a
    jello like gel

5
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