Discussion 7

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Discussion 7

• Glycosphingolipids (GSLs)Glycosylphosphatidylinos
  itol (GPI) Anchors

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N-linked Type Oligosaccharides
High Mannose
Hybrid
Complex
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O-linked Mucin-Type Oligosaccharides
Sialyl Tn antigen
T antigen
Tn antigen
Branched
Keratan Sulfate
Core 1
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Sulfatide, a galactosylceramide
GT1b, a ganglioside
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Major Core Structures
Lacto Lc4Cer
NeoLacto nLc4Cer
Ganglio Gg4Cer
Globo Gb4Cer
isoGlobo iGb4Cer
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Common Glycan Epitopes
b3
b4
b4
b4
b3
b6
b4
b4
b3
b4
a3
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Blood Group Epitopes
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Lewis Antigens
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Glycosphingolipids
H or O
Blood Group B
Blood Group A
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Microdomains
CD59
Sph
GSL
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Uptake by Endocytosis
Pinocytosis
Recycling Endosome
Clathrin-Mediated Endocytosis
Early Endosome
Sorting Endosome
Sphingosine Fatty Acids Monosaccharides
Ligand
Lysosome
Glycolipid
Clathrin/AP-2
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Arthropods
Manb4Glc-Cer
GlcA
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PNH (Paroxysmal Nocturnal Hemoglobinuria) and GPI
anchors

• PNH is caused by a defect in GPI anchor formation
  (PigA).
• A number of surface proteins are unable to remain
  tethered to the cell surface
• Loss of CD55, also called Decay Accelerating
  Factor (DAF), and CD59, also called Membrane
  Inhibitor of Complement Lysis (MIRL).
• Enhanced sensitivity to complement-mediated cell
  destruction.
• Red blood cell, white blood cell, and platelet
  counts may be low.
• Urine may be intermittently red or brown,
  signifying the breakdown of red blood cells with
  release of hemoglobin