Cytosolic

1
RyR2 open probability
Iso
CHF
ECC gain Ca transient contractility
Ca
DADs - SCD
Cytosolic Ca
Time
2
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3
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4
IP3R RyR
Skel muscle
Smooth m.
Neurons
IP3 Activates None
Ryanodine None Locks open/closes
Caffeine (5 mM) Inhibits Activates
Ca2 IP3R1 - biphasic IP3R2/3 - opens biphasic
RR None inhibits
Heparin inhibits activates
5
Excitation-secretion coupling Inositol
1,4,5-trisphosphate receptors
6
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7
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8
IP3 signaling
9
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10
Stable transfectant Jurkat T cells do
not express IP3R1
IP3R1
11
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12
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13
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14
Activation of IP3-gated Ca2 channel by fyn
15
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16
Calcium signaling during apoptosis
Growth Factor
Plasma Membrane
PIP2
Rec
PLC-
g
Cytoplasm
P
IP3
14-3-3
P
2
ER
Bad
Ca
Bad
B
c
l
-
2
Degradation
IP3R
S
ERCA
2
Akt
Ca
X ?
Calcineurin
Cytosolic T
F
B
c
l
-
2
B
c
l
-
2
B
c
l
-
2
Bad
DNA
CAD
Bcl-x
Digestion
Nuclear
Caspases
TF
M
M
Cytochrome C
Nucleus
Cytoskeletal Breakdown
Survival
Survival
Cell Death
17
NCE
mM Ca
mitochondria
nAChR
CNG
VDAC
Stretch receptor
Endoplasmic reticulum
mM Ca
PMCA
IP3R
SERCA
100 nM Ca
T-tubule
mM Ca
Sarcoplasmic reticulum
VGCC
RyR
SERCA