Sample Storage

1
Sample Storage

• If coagulation testing (other than PT) can not be
  performed within the 4 hour window from specimen
  acquisition to analysis, it is critical that
  platelet poor plasma (PPP) be properly stored.
  Aliquots of PPP should be frozen immediately
  after processing and not held at 4 oC. If
  samples are stored at 20 oC, it is important
  that a non-frost-free freezer (manual) be used
  since automatic defrost cycles may cause sample
  deterioration during the heating phase. For
  example, FVIII activity can DECREASE by 20 after
  4 hours at 4 oC, by 34 when stored at -20 oC
  (cycling freezer), and by 11 at 70 oC. The
  activity of FV, the other labile factor can
  decrease by 20 at -20 oC. We recommend that all
  referred samples be stored at -70 oC.
• Personal Communication Pamela Fisher, MT(ASCP),
  The Mayo Clinic, Rochester, MN

Lab Pointer
? FV ?
? FV ?
? FVIII ?
? FVIII ?
Manual Defrost Freezer
www.CLOT-ED.com
M Ledford-Kraemer
Revised mmiii