Lateral Plate Mesoderm and Endoderm

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Lateral Plate Mesoderm and Endoderm

• Heart
• Blood and blood vessels
• Pharynx
• Liver
• Pancreas
• Lungs
• Extraembryonic membranes
• Chorion
• Amnion
• Yolk sac
• allantois

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Cardiogenic mesoderm
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Harmonious equipotential system (Driesch)
Anterior Intestinal Portal
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• Embryonic circuit
• yolk sac capillaries
• vitelline vein
• sinus venosus
• atrium
• ventricle
• conotruncus
• atrial sac
• ventral aorta
• aortic arches
• dorsal aorta
• vitelline arteries
• yolk sac capillaries

Why are these colors wrong?
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Chorioallantoic placenta
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10 mm pig pharynx, larynx, esophagus, trachea,
lung buds
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Experiments (find it, lose/block it, move it)

• Observe location of pancreas formation. (Fig 1)
• Combine endoderm and aortae in vitro. (Fig 2 and
  Table 1)
• Observe effects of aorta defects in vivo. (Fig 3)
• Observe effects of ectopic vascularization on gut
  using transgenic mouse. (Figs 4 and 5).
• http//www.sciencemag.org/cgi/content/full/10
  64344/DC1

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Glossary

• VEGF. Vascular endothelial growth factor
  (paracrine factor).
• VEGFR. Receptor for VEGF.
• PECAM1. Platelet endothelial-cell adhesion
  molecule. A marker for blood vessels.

• Pdx1. Transcription factor a pancreas marker.
• Lac Z staining. Reporter gene inserted into Pdx1
  gene to make visualization of Pdx1 expression
  easier.
• NeuroD and Pax6. Pancreas TF mRNAs.
• Insulin and PP. Protein markers for pancreas.

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• Chorion
• Amnion
• Yolk sac
• Allantois