Old World Climbing Fern (Lygodium microphyllum)

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Old World Climbing Fern (Lygodium microphyllum)
DEP 2007
DEP Funded Research

• Jeff Hutchinson and Dr. Ken Langeland
• University of Florida, Department of Agronomy
• Center for Aquatic and Invasive Plants

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1. Herbicide Translocation
2. Herbicide Trialsa. Greenhouseb. Field
3. Herbicide Resistance

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Herbicide Translocation Radio-labeled 14C
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5 Treatments

• 3 Herbicides
• Glyphosate
• Metsulfuronmethyl
• Triclopyr

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Triclopyr
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Metsulfuron methyl
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(No Transcript)
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Greenhouse Herbicide Trials (2005-2006)

• Glyphosate (Aquapro) - 7 ½ pt/ac
• Metsulfuron methyl (Escort XP) - 2 oz/ac
• Imazapyr (Habitat) - 6 pt/ac
• Imazapic (Plateau) -12 oz/ac
• Quinclorac (Drive) - 32 oz/ac
• Penoxsulam (Grasp) -2.8 oz/ac
• Imazamox (Raptor) - 6.0 oz/ac
• Triclopyr (Renovate 3) - 8 qt/ac

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Glyphosate
Quinclorac
Triclopyr
2 Weeks Post-Treatment
14 Weeks Post-Treatment
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Dry-Weight Biomass (5 months post-treatment)
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Additional Herbicide Trials (2006 2007)

• Aminopyralid (Milestone)? 0.875, 1.75, 3.50, and
  7.00 oz / acre
• Fluroxypyr (Vista)? 5.25, 10.5, 21.0, and 42.0
  oz / acre
• Flumioxazin (Sureguard)? 1.5, 3.0, 6.0, and 12.0
  oz / acre
• Bispyribac-sodium (Regiment)? 0.33, 0.66, 1.33,
  and 2.65 oz / acre
• Imazomax (Clearcast, higher rates)? 6.4, 12.8,
  25.6, and 51.2 oz / acre

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8 Weeks Post-treatment
Imazamox
Fluroxypyr
20 Weeks Post-treatment- No re-growth for
imazamox at 25.6 and 51.2 oz/ac
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Field Herbicide Trials(2006-2008)
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Herbicide Rates

• Single Herbicides
• Glyphosate 2.0
• Glyphosate 4.0
• Metsulfuron methyl 2 oz/100 gallons
• Metsulfuron methyl 4 oz/100 gallons
• Triclopyr 2.0
• Imazapic 1.5

• Tank Mixes
• Metsulfuron 2oz Glyphosate 2
• Metsulfuron 2oz Triclopyr 2
• Metsulfuron 2oz Imazapic 1.5
• Glyphosate 2 Triclopyr 2
• Glyphosate 2 Imazapic 1.5
• Glyphosate 1 Triclopyr 1 Metsulfuron 1oz

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Metsulfuron 2oz Plateau 1.5 (IRCC)
Imazapic 1.5 (JDSP)
Pre-Trt
6 months
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Herbicide Resistance

• Germination Rates
• Spore samples from gt 10 populations
• 100 spores (n 10) from each sample
• Place in agar media
• Count germinating spores

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Spore Counts
1) Weigh out known amount of spores 0.03
grams 2) Suspend in 1 ml of water 3) Take 0.1 ul
sample and count with Haemocytometer 4) Spores
estimates - 289200 spores / 0.03 g / 1 ml water
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Metsulfuron methyl(exposure rates)

1. Expose spores in nutrient / agar herbicide
   solution
2. Determine resistancerate
3. Allow resistancebiotype to matureand collect
   spores
4. Repeat experiment

• 0.05 oz
• 0.10 oz
• 0.25 oz
• 0.50 oz
• 1.00 oz

• 1.50 oz
• 2.00 oz
• 3.00 oz
• 4.00 oz
• 8.00 oz

(at 20 and 100 gal/ac)
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Conclusions

• Translocation work indicates that triclopyr
  translocates significantly better than glyphosate
  and metsulfuron.
• Glyphosate, metsulfuron methyl, and triclopyr
  provided excellent control of L. microphyllum
  under greenhouse trials.
• In field trials, tanks mixes provided better
  control than single herbicides, but results are
  preliminary.
• Investigations on the ability of L. microphyllum
  to exhibit resistance to metsulfuron methyl are
  being conducted