Poster Presentation

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Poster Presentation
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Make The Title Brief And Descriptive.

• Continuing Efforts to Exploit Third Strand
  Binding for Repair of the Sickle Cell Mutation in
  Human Cell Lines

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Provide A Brief Abstract

• To utilize short homopyrimidine deoxyoligomers as
  highly specific third strand binding agents for
  delivery of reagents to modify mutant residues at
  unique sites in the human genome, we have
  explored methods for transfecting them into HeLa
  and K562 cells, and demonstrated that they do
  bind to the correct target sequences. This work
  is part of an effort to develop a method for
  correcting point mutations responsible for
  diseases such as that in the ?-globin gene of
  Sickle Cell Anemia. While transfection of linear
  and plasmid DNA into human cells is
  well-established, this is not so for single
  strands 20-40 residues long, as required for gene
  mutation correction. After exploring many
  transfection reagents, one was found that is
  effective and non-toxic. To monitor their entry
  into cells, the strands were labeled with the
  fluors FITC or TMR. Probe strands with 5
  psoralen and 3 fluor termini were transfected
  into cells, and observed to enter nuclei rapidly.
  After 23 hours, colchicine was added to halt cell
  progression in metaphase an hour later UVA light
  was used to covalently link the probes wherever
  they might be bound. After bursting the cells in
  hypotonic solution, their chromosomes were spread
  on slides and examined by confocal microscopy.
  Using probes specific to centromeric sequences of
  chromosomes 11 and 17, and to the ?-globin gene
  in chromosome 11 and the Her2/neu and Topo2 genes
  in chromosome 17, highly specific probe binding
  to the correct chromosome centromeric and arm
  regions was observed. Transfection kinetics and
  efficiency were determined using fluorescence
  activated cell sorting.

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Plan A Story For The Viewer

• the context - what, why, how
•  the results and analysis 
• the importance of the results

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• An effective poster is ...
• Focused Focused on a single message.
• Graphic Lets graphs and images tell the story
  uses text sparingly.
• Ordered Keeps the sequence well-ordered and
  obvious.

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Academic Study

• How do you get acceptance
• USA
• Germany
• Israil
• Holland
• EU Universities or inst.

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Exams and Others

• GPA
• GRE
• TOEFL (IBT)
• CV
• SAP
• References
• Publication

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What can you be after PhD

• Dr.
• Assist. Prof
• Assoc. Prof
• Prof.