The New age Shower

1
The New age Shower

• James Diewald
• Michelle Cisar
• Chris Jones
• Paul Georgadis

2
The Experiment

• Bacteria are everywhere.
• After collecting and identifying bacteria
  collected from the entire western campus we
  decided to narrow our search to one bathroom.
• That bathroom is 3 South Peabody Hall.
• Samples will be taken from designated sights in
  the bathroom.

3
The Essential Background

• Most domestic bacteria ar harmless but some rare
  strains can cause death.
• Bacteria are the most common organisms on earth.
• Bacteria are 80 water.
• Every one of you has bacteria inside of you
• Bacteria prosper in warm moist places, preferably
  between 25 and 35 degrees C (your body
  temperature).
• Your bacteria are good for you.
• High volume bathrooms are bacteria breeding
  grounds.

4
What you Need to Know

• The decided sampling locations are as follows.
• 1. The toilet seat, flush handle and outgoing
  door latch.
• 2. The outgoing door handle.
• 3. The sink bowl and knobs.
• 4. The shower floor and knobs.

• We expect a number of bacteria to be thriving at
  these prescribed locations.
• All of which are thought to be infectious to
  humans and some may be deadly.

5
The Suspects

• The cell wall is composed of peptidoglycan, a
  protein-sugar molecule.
• No conventional nucleus.
• Uses flagellum for movement.

6
The Suspects (1)

• Staphylococci
• -yellow, spherical cells in irregular clusters
• -carried by 15-20 of population
• -common infections
• hair follicle, bone, intestine, urinary tract

7
The Suspects (2)

• Streptococci
• -small white, semi-transparent, chains of
  spherical cells
• -very common
• -common infections
• throat, skin, joints, heart

8
The Suspects (3)

• E-coli
• -smooth, orange, spherical cells
• -part of normal flora of intestinal tract
• -common infections
• intestines, travelers diarrhea, fatal
  cholera-like disease

9
The Suspects (4)

• Salmonella
• -larger, tan, smooth cells
• -transmitted through contaminated food and water
• -common infections
• gastrointestinal tract, abdomen

10
The Suspects (5)

• Campylobactor
• -white, curved rods
• -ranks with E-coli as major cause of diarrhea in
  world
• -common infections
• intestinal tract, diarrhea

11
Further Expectations

• We expect the shower and the door handle to have
  the highest levels of bacteria.
• The shower bacteria levels will be high due to
  traffic and moisture levels that advocate
  bacteria growth.

• The door handle is the single most touched object
  in the bathroom and may therefore have high
  levels of bacteria.
• We also expect a significant difference before
  and after cleaning.

12
Materials

• 16 Agar Plates
• Cotton Swabs
• Permanent Marker
• Tape (to seal agar after sampling)

13
Sampling Procedures

• We took before and after cleaning samples on each
  of two days from the named locations.
• A specific Circle X plating method was created
  in order to insure a uniform bacteria
  distribution on the agar plates.

• To calculate the percent coverage of each
  species, we utilized Photoshop to lay a grid over
  a series of digital photos.
• The photo was zoomed in on and the percentage was
  established.

14
Mission Results

• As before, bacteria are everywhere.
• The following graphs will provide detailed data
  on our results.

15
Debrief (1)
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Debrief (2)
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Debrief (3)
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Debrief (4)
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Debrief (5)
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Debrief (6)
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Debrief (7)
22
Final Conclusions

• Statistical analysis via Statview showed no
  significant difference before and after cleaning.
• Our graphs did show otherwise.
• We assume that a larger data set would yeild our
  predicted results.