Glutamate Monitoring in Brain Interference Elimination

1
Glutamate Monitoring in Brain - Interference
Elimination

• A. Collins 1, E. Mikeladze 2, T. Laurell 3 and E.
  Csöregi 1
• 1 Department of Biotechnology, Lund University,
  Lund, Sweden
• 2 Biochemical Neuropharmacology, Institute of
  Physiology, Tbilisi, Georgia
• 3 Department of Electrical Measurements, Lund
  University, Lund, Sweden

Glutamate Biosensor
Endogeneous Interferents
Biological Relevance

• Electrochemically active compounds (e.g.
  ascorbate, urate, acetaminophen)
• Oxidizable at the applied potential -50 mV vs.
  Ag/AgCl
• Major interferent ascorbate, AAbrain 500 µM
• Elimination Possibilities
• Preoxidizing cell
• Use of various membranes (coating the sensor)
• Enzymatic preoxidation (e.g. enzyme
  microreactors, enzymes integrated in biosensor
  design)

• L-glutamate is one of the major excitatory
• neurotransmitters in the brain
• Overstimulation results in neuronal cell death
• Glutamate receptor hypofunction in Schizophrenia
• Glutamate-blocking compounds in stroke treatment
• Linked to Alzheimers and Parkinsons
• Complex actions in the brain acts at multiple
  receptors (ionotropic, metabotropic), enabling
  the design of
• various pharmacological drugs