Workpackage 0 (WP0): Management of project

1
Workpackage 0 (WP0) Management of project

• Objectives
• Project management, supervision of sample
  distribution and exchange of material between
  partners
• Confirmation of deliverables and milestones
• Submission of reports, supervision of periodic
  meetings and budget administration
• Supervision of websites formation and
  availability

2
Workpackage 0 (WP0) Management of project

• Description of works
• Coordination of research activities by partners,
  flow of samples and data interaction between
  partners
• Supervision of two websites
• a) Public with privileged access to partners
  (contractor)
• b) Platform for data (P5)
• 3. Supervision of synthesis of genomic,
  proteomic and metabolomic data
• Organization of semi-annual and annual meetings
• Integration and dissemination of results and
  maintaining contacts with the EC (Dr. C.
  Krassnig)
• Responsibility for all contractual activities,
  including intellectual properties

3
Workpackage 0 (WP0) Management of project
Deliverable title Deliverable Number Deliverable Date
Biennial and annual meetings Short biennial reports and two periodic reports at M18 and M36 D1 M6, M12, M18, M24, M30, M36
Formation of the public accessible project website D2 M12
Distribution of results and samples between partners D3 M36
4
Workpackage 1 (WP1) Mechanisms for establishment
of dormant stage

• Objectives
• Characterize and optimize conditions for
  obtaining akinetes in culture (P1 Ora Assaf)
• 2. Identification of genes, proteins and
  compounds associated with formation of akinetes
  in cyanobacteria and resting eggs in rotifers
  (P1, P5, P6 Ora, Assaf, Esther, Richard and
  Jens)
• 3. Studies on yeast sporulation and establishment
  of the dormant stage (P3, P5, P6 Stefan, Richard
  Jens)

5
WP1 Description of work

• The effect of environmental factors on akinetes
  formation (P1 Ora Assaf)
• 2. Rotifer cultures and resting egg production
  (P1 Esther)
• 3. cDNA libraries and microarrays (Richard in
  samples supplied by Ora, Assaf Esther)
• 4. Temporal expression of genes that are up-
  regulated or down-regulated during the formation
  of akinetes and resting eggs (Ora, Assaf, Esther
  Richard)
• 5. Search for proteins and compounds (Richard and
  Jens on samples supplied by Assaf, Ora, Esther
  Stefan)
• 6. Studies on sporulation and establishment of
  the dormant stage (Stefan)

6
Workpackage 1 Deliverables
Deliverables Deliverable Number Deliverable Date
Optimal conditions for inducing of akinetes asexually and sexually reproducing rotifers and their resting eggs Ora, Assaf Esther D4 M9
cDNA libraries, EST sequencing and database construction, including a website accessible to Partners - Richard D5 M16
Pattern of global gene expression during formation of akinetes, rotifer resting eggs by microarray analysis Ora, Assaf, Esther Richard D6 M27
Quantitative analyses of the expression of specific genes by QPCR Ora, Assaf Esther D7 M35
D8 M36
7
Workpackage 1 Deliverables (Continued)
Deliverables Deliverable Number Deliverable date
Proteins and metabolites identified to be associated with formation of resting eggs or contained within them Richard, Jens, Ora, Assaf Esther D8 M30
Genes, proteins and metabolites associated with the dormant stages of yeast spores Stefan, Richard Jens D9 M36
8
Movement of samples - M6

• To Richard
• 1) Ora, Assaf, Esther samples for
  construction of libraries and sequencing
• (to be discussed)
• 2) Stefan Samples for Proteome
  analyses (?)
• To Jens None

9
Workpackage 6 (WP6)Dissemination of results

• Objectives and description of work
• Dissemination of results by participation in
  scientific meetings
• The organization of a workshop on desiccation
  stress and dormancy
• Publication of a book on desiccation stress and
  dormancy

10
Workpackage 6 (WP6)Dissemination of results
Deliverables Deliverable Number Deliverable date
Participation in scientific meetings D28 M36
A workshop on dormancy opportunities and challenges preservation of cells and organisms D29 M24
Publication of a book on dormancy D30 M36
To be decided at the meeting