BS111L Lab Final Review

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BS111L Lab Final Review

• Exam Dates, Times Locations
• Sections 4 to 7 December 14 (Tuesday)
• at 3 5 pm in 109 SKH
• December 16 (Thursday) at 3 5 pm
• - Sections 1 to 3 in 105 SKH
• - Sections 8 to 10 in 107 SKH
• Sections 11 - 14 December 17 (Friday) at 745
  945 am in 105 SKH

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Formulas

• Calculate in Common between two species
• number of proteins in common between both species
• --------------------------------------------------
  -------------------------
• total number of different proteins present in
  both species

X 100
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Dilution -Formulas

• Manipulate the formulas (How set up dilution if
  you want to make 10 mls for each dilution? )
• C1V1 C2V2 and V2 V1 Vd
• C1_V1_ C2
• V2
• __V1__ __V1__
• V2 V1 Vd

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Total Magnification
Mtotal Mobjective X Mocular
For compound and dissecting microscopes
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Metabolism
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Biological Molecules (Parts II IV)
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BS111L Recitation

• Graphing (page 33 34)
• Standard curve

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Protein Concentration

• 4. Results

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Colloid Analysis

• 4. Results

y-axis can label as Molecular Weight (g/mole)
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Enzymes Environmental Analysis

• 4. Results

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Enzymes Environmental Analysis

• 4. Results

Acid hydrolysis
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Enzymes Environmental Analysis

• 4. Results

Substrate limiting
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Protein Fingerprinting Analysis

• 4. Results Determining sizes of unknown proteins?

Catfish Protein 26 mm So how Big?
catfish
50 kD
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Standard Curve for Molecular Lab
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Organismal Respiration
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Glycolysis Fermentation
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Diffusion and Osmosis


Solute
Solvent (H20)
In which direction is the solute moving?
In which direction is the water moving?
Versus Other Side of Membrane
Versus Other Side of Membrane
Lower Osmotic Pressure
Higher Osmotic Pressure
Semipermeable Membrane
Hypotonic
Hypertonic
Lower Total Solute Concentration
Higher Total Solute Concentration
Higher Water Potential / Free water
Lower Water Potential / Free water
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Cells in a
Hypertonic solution high conc solute / low
solvent (H2O) conc.
Hypotonic solution low conc solute / high
solvent (H2O) conc.
Isotonic solution same conc solute / same
solvent (H2O) conc.
crenated
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Protein Fingerprinting Analysis

• Results Calculate in Common between most
  similar related species salmon cod

AM Fl Co Sa K Ca Sc
MHC A T MLC MLC
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Maps
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Lane 1 linear Lane 2 linear Standard Lane 3
linear Lane 4 nicked and supercoiled Lane 5
linear
nicked
supercoiled
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Station 4 electrophoresis, page 20

• Separating out different sizes (mass or molecular
  weight) of proteins in SDS-PAGE (polyacrylamide
  gel)

• proteins must have a negative charge
• Be soluble in solution
• Be denatured (linearizes)

cathode
- Smaller protein chains move farther distances
than larger protein chains
Anode
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Station 4 Gel electrophoresis, page 20

• Separating out DNA fragments in 0.8 agarose due
  to differences in size (base pairs) and shape

• DNA must have a negative
• charge
• Be soluble in solution
• Different shapes (linear,
• supercoiled, nicked relaxed)

• larger linear fragments move
• less distance than smaller linear
• fragments

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Station 3 Measuring page 17
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Station 3 Measuring page 17
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Station 3 Measuring page 17
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Microscopy, page 32
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Microscopy, page 32