The Microscope

1
The Microscope
2
The Microscope

• Spectroscopic Microscope
• Light Compound (Bright field)
• Fluorescent
• Confocal
• Electron- Transmission and scanning

3
Fluorescence Microscopy
Confocal Microscopy
4
Illuminating system

• Light source
• Condenser lens- immediately below specimen
• Iris diaphragm- controls amount of light

5
Imaging system

• Objectives-4X, 10X, 40X
• Ocular- lens to look through monocular
  binocular
• Oculars magnify 10 times
• Stage- place where specimen are mounted

6
Magnification

• Magnification total Mag obj X Mag ocu
• Parfocal
• Parcentered
• Field of view

7
Stages of making a slide

• Sectioning-blade
• Mounting-(wet)- glycerin water
• Dehydration-alcohol
• Fixing-formaldehyde glutaraldehyde

8
Determining size of field

• Micrometers
• Ocular micrometer
• Stage micrometer
• FOV

9
Use of the micrometer
Demonstration