EURON course Advanced Microscopy

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EURON courseAdvanced MicroscopyVital Imaging
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Immunofluorescence microscopy microfilament
bundles
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Live cell imaging Mitosis
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ModernTechnologies

• Recombinant DNA technology and vital probes
  e.g.Green Fluorescent Protein (constructs)
• Advanced microscopical technologies Confocal
  Laser Scanning microscopy Multiphoton Laser
  Scanning microscopy
• Advanced image reconstruction programmes

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TPLSM at UM and BME
Overview of the set-up at the UM
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3-D reconstruction of neurons after two-photon SLM
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3-D reconstruction of neurons after two-photon
CSLM
Zeiss
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Projection of Z-series
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Vital imaging using Green Fluorescent Protein
(GFP)
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Microtubule spindle during mitosis
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Collaborating microtubules and microfilaments in
neurite formation
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Vital imaging of microfilaments in growth cones
Keach et al.
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Vital imaging of microtubule dynamics
Ludin et al.
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Nuclear lamina 3D in time
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Lamina during cell cycle
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Cyclin B during the cell cycle
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Apoptose
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Two-photon confocal scanning laser microscopy
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Z-series of lamin fluorescence
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Living cells transfected with lamin-GFP