Benefits to Cadence of the MIT MAP-Chip Project

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Carlson, Pace Proliferation of Biological
Technologies, Biosec. Bioterror. 1(3)1 (2003)
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Struggle, Limited Success, Struggle
Devices?
System??
Design Fabrication
Application
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Struggle, Success, Predictable Success
Applications
Systems
Parts Fabrication
Design
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Enabling Biological Engineering

• Decoupling
• Rules insulating design process from details of
  fabrication
• Enable parts, device, and system designers to
  work together
• VLSI electronics, 1970s
• Standardization
• Predictable performance
• Off-the-shelf
• ME, 1800s
• Abstraction
• Insulate relevant characteristics from
  overwhelming detail
• Simple artifacts that can be used in combination
• From Physics to EE, 1800s

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Abstraction
Systems
Devices
Parts
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Parts
Zif268, Paveltich Pabo c. 1991
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Devices
CI
LacI
l cI-857
OLac
RBS
T
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Devices
LacI ? CI inverter
LacI
CI
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Systems
Inverter.2
Inverter.3
Inverter.1
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Interfaces
Systems
Inv.2
Inv.3
Inv.1
LacI ? CI inverter
LacI
CI
Devices
Parts
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Parts/Device Interface
LacI ? CI inverter
LacI
CI
Devices
Parts
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Stories
In 1910, I was in Mexico, in the state of
Yucatan, when an invasion of locusts occured the
Indians reported to me that in a certain place
the ground was strewn with the corpses of these
insects. I went there and collected sick
locusts, easily picked out since their principal
symptom was an abundant blackish diarrhoea. This
malady had not as yet been described, so I
studied it. It was a septicaemia with intestinal
symptoms, It was caused by bacteria, the locust
coccobacilli, which were present almost in the
pure state in the diarrhoeal liquid. I could
start epidemics in columns of healthy insects by
dusting cultures of the coccobacillus on plants
in front of the advancing columns the insects
infected themselves as they devoured the soiled
plants In the course of these researches, at
various times I noticed an anomaly, shown by some
cultures of the coccocacillus which intrigued me
greatly, although in fact the observation was
ordinary enough, so banal indeed that many
bacteriologists had certainly made it before on a
variety of cultures. The anomaly consisted of
clear spots, quite circular, two or three
millimeters in diameter, speckling the cultures
grown on agar. From The Bacteriophage by Dr.
Felix d'Herelle, Science News 14 44-59 (1949).
(Translation by J. L. Crammer)
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Parts/Device Interface
LacI ? CI inverter
LacI
CI
Devices
X
X
Parts
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Device/System Interface
Inv.2
Inv.3
Inv.1
Systems
Devices
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Device/System Interface
X
E ? F
C ? D
A ? B
X
X
Systems
Devices
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Device/System Interface
A ? D
E ? F
C ? D
A ? B
X
X
Systems
X
Devices
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Device/System Interface
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Polymerase Per Second PoPS!
T
l cI
RBS
Ol
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Polymerase Per Second PoPS!
PoPSOUT
PoPSIN
T
l cI
RBS
Ol
cI
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Device/System Interface
Systems
X
Devices
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Device/System Interface
Systems
X
A
PoPSOUT
Devices
PoPSIN
B
PoPSOUT
PoPSIN
C
PoPSIN
PoPSOUT
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(No Transcript)
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Device-Level System Diagram
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Parts- and Device-Level System Diagram
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DNA Layout
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Characterization and Debug
Trigger Test Circuit
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Registry of Standard Biological Parts
http//parts.mit.edu/
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From XXXX Subject Endy Letter Date January 6,
2005 94517 AM EST To endy_at_mit.edu Dr. Endy,
I am a sophomore at XXXXX High School in
Connecticut and have recently taken an interest
in Synthetic Biology.I am writing to ask for your
help because i am having difficulty in obtaining
information,and understanding some of the
information i already have. Anything you can send
my way would be greatly appreciated I will
soon begin working on a proposal to create a
BioBrick, any information you can send me on
their creation would be excellent. -Sincerely,
XXXX XXXX XXXX High School -Grade 10
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Education Driving Research
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A Constructive Society
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UT 2004 SB Competition Team
c/o Jeff Tabor
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UT 2004 SB Competition Team
Light ? PoPS Receiver
BBa_I15010 BBa_R0082
Photons
PoPS ? Color Converter
BBa_B0034 BBa_E0033 BBa_B0015
PoPS
c/o Jeff Tabor
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UT 2004 SB Competition Team
Lens ripped off of overhead projector
Pile of cells/agar
Casserole dish
Thermostable chassis
c/o Jeff Tabor
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UT 2004 SB Competition Team
c/o Jeff Tabor