QVirus Platform

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QVirusTM Platform
Our platform offers a comprehensive portfolio of
services for the efficient design, development,
manufacturing and analytical testing of virus
vector products.
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An Overview of QVirusTM Platform
Viral vectors have been widely used as gene
delivery vehicles. Compared to the conventional
transfection methods, transduction usually
results in much higher gene delivery efficiency,
sometimes almost 100. However, construction of a
viral vector is a laborious process, and most
commercial services in this area have so far been
either too expensive or not user-friendly. With
years of experience, Creative Biogene has
launched a powerful QVirusTM Platform for
Lentivirus, Adeno-associated Virus (AAV),
Adenoviruses, Retrovirus, Oncolytic virus, etc.
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QVirusTM Platform Working Principle
In order to produce viruses with alternate
(non-virus-producing) genomes, naturally
occurring viral genomes have been adapted into a
plasmid-based technology, such that plasmids can
be used to create viruses with specific genomes
(Figure. 1A). That is to say, instead of a virus
infecting a host and giving rise to more viruses,
researchers can introduce plasmids to a host to
generate virus. Moreover, these plasmids can be
modified to give rise to viral genomes of choice
(Figure. 1B). Therefore, through standard plasmid
cloning, viruses can be engineered to harbor a
lot of viral genomes, enabling researchers to
direct genetic functions widely in cells.
Figure 1. Using viral plasmids to generate virus.
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QVirusTM Platform Multiple Viral Vectors
Available
There are various types of viruses which are
commonly used for research, each of which
exhibits different properties, and therefore, are
suited for specific research goals. For instance,
AAV is typically preferred for in vivo studies
owing to its low immunogenicity. Different types
of viruses also vary in the composition of their
viral genomes, with some having an RNA genome
while others have a DNA genome. Furthermore, some
viruses function by permanently integrating into
the host's genome while other viruses are
temporary in the host.
Our QVirusTM Platform has developed a number of
viruses, including lentivirus, AAV, adenovirus
and retrovirus, etc.
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QVirusTM Platform Multiple Viral Vectors
Available
The charateristics of different viruses as
follows
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QVirusTM Platform Viral Vector Construction
Our QVirusTM Platform offers a variety of
optimized viral vectors for your applications. We
can subclone your gene of interest into the viral
vectors with custom choices of promoters, tags,
fusions, and selectable markers under different
regulatory elements.
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QVirusTM Platform Virus Packaging
Viral vectors are one of the most efficient
methods of gene delivery to mammalian cells both
in vitro and in vivo. Through years of experience
with viral vectors, our QVirusTM Platform has
developed its own proprietary virus packaging
systems and efficient protocols for the rapid
generation of pseudoviral particles. The viral
expression constructs packaged into pseudoviral
particles can be transduced into cells with very
high efficiency, approaching 100 in some cell
types. Packaged viral constructs can be
transduced into even the most difficult to
transfect cells, such as primary, stem, and
differentiated cells with high efficiency.
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QVirusTM Platform Virus Packaging
Our QVirusTM Platform offers packaging services
for the following virus types
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QVirusTM Platform Virus Purification and
Titration
The highly purified virus is a requirement for a
lot of downstream applications since cellular
debris and proteins derived from culture media in
crude virus preparation can be toxic to target
cells and can cause immunogenic reactions when
used for in vivo injection. Using purified virus
stocks also prevents pseudo-transduction, in
which high levels of recombinant protein in the
crude supernatant are passively transferred to
target cells during infection.
With years of experience in research of virus,
Creative Biogene is able to produce high purity
and high titer virus that is suitable for both in
vitro cell experiments and in vivo animal tests.
Our QVirusTM Platform can help you to remove
cellular contaminants, including uncharacterized
infection and transduction inhibitors, residual
plasmid DNA, cellular and serum proteins, and
highly immunogenic viral proteins, nucleic acids,
and virus fragments.
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QVirusTM Platform Application
Viral vectors are tools commonly used by
molecular biologists to deliver genetic material
into cells.
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QVirusTM Platform Advantages
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THANK YOU
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