Prime IVF Centre (1)

1
Prime IVF
2
What Is Preimplantation Genetic Diagnosis (PGD)?

• Some of the commonly diagnosed disorders by a
  preimplantation genetic diagnosis in Delhi and
  elsewhere include
• Reproductive cancer-causing BRCA1 BRCA2 gene
  mutations,
• Cystic fibrosis (CF),
• Sickle cell anemia,
• Hemophilia A,

3
Why Is It Performed?

• PGD focuses on mitigating the risk of passing a
  genetic defect or chromosomal anomalies to the
  child. PGD is preferred and performed during an
  IVF, especially in the following cases.
• Intended parents with a family history of
  X-linked disorders like fragile X syndrome
• Couples who have had recurrent pregnancy loss or
  implantation failures due to chromosomal defects
  of the embryo
• Parents who are carriers for an autosomal
  dominant, recessive condition like sickle cell
  disease, Huntington's disease, or other X-linked
  disorders
• The risk of passing on the disorder for autosomal
  recessive is 25 but, in the case of the
  autosomal dominant condition, it is 50.

4
What is the Procedure of Preimplantation Genetic
Diagnosis (PGD)?

• The procedure of PGD is performed during an IVF.
  Once the genetic counselor or a geneticist
  assesses the risk of passing on the genetic
  condition to their offspring, the following steps
  of IVF are followed by embryo biopsy procedures
  to derive embryo cell samples for testing and
  genetic testing procedures.

5
Embryo biopsy techniques in PGD

• Cleavage stage embryo biopsy- At day three of
  fertilization, a small blastomere cell of 6-10
  cell stage embryos is extracted for testing.
• Blastocyst biopsy- The blastocyst stage embryo
  has more than 100 cells defined by outer and
  inner cell mass. Cells from the outer cell mass
  or trophectoderm are extracted for further
  genetic testing.

6
Genetic testing procedures in PGD

• Polymerase chain reaction (PCR) PCR is a
  relatively faster method of DNA testing where a
  strand of DNA is amplified to check for genetic
  mutations in single-gene disorders, autosomal
  dominant, and recessive disorders.
• Fluorescent in situ Hybridization (FISH) FISH is
  usually considered for X-linked disorders,
  chromosomal aberrations, and aneuploidy
  screening. Here, fluorescent markers that bind to
  specific mutated chromosomal segments are used to
  analyze the DNA.

7
Contact us

• Business Name- Prime IVF Centre
• Address- 4636 B, Near Columbia,Asia hospital,
  Sec 23A ,Gurgaon,Haryana 122017
• Phone- 9026869869
• E-mail- info_at_primeivfcentre.com
• Website- https//www.primeivfcentre.com/