T cell activation assay

1
T Cell Activation assay

• https//www.creative-biolabs.com/car-t/

2
As an excellent service leader in the field of
antibody development and immunotherapy, Creative
Biolabs now offers one-stop CAR-T therapy
development service which has become a promising
approach to antitumor therapy. Antigen specific T
cell activation assay is one of the technologies
we can provide during the one-stop service which
can activate CAR-T cells for further downstream
research or clinical trials.
3
Background
T cell activation requires at least two signals
to be fully activated. The first signal is
provided by the interaction of T cell
antigen-specific receptor and the antigen-major
histocompatibility complex (MHC). The second
signal is provided by co-stimulatory receptors
like CD28. In engineered CAR-T cell, CAR consists
of specific scFv and signaling endodomains of
CD3?, CD28, and 4-1BB. Creative Biolabs offers
one-stop CAR-T service from basic research to
clinical trials and beyond, and antigen specific
T cell activation assay has been a vital
technology in evaluating the activation
activities of different CAR-T cell products at
preclinical stage.
4

Assay of Antigen Specific T Cell Activation
CAR-modified T cells can recognize tumor cells
via binding of the CAR to its tumor-associated
antigen (TAA) independent of TCR-MHC/peptide
interactions, and the engineered signaling
endodomains can help T cells be activated and
persistent. As a result, T cells are activated
and thus they can efficiently eliminate tumor
cells by the secretion of perforin and granzymes
as well as the expression of FasL and tumor
necrosis factor-related apoptosis inducing ligand
(TRAIL). In addition, other tumor-infiltrating
immune cells can be activated by the secretion of
various cytokines.
5
Creative Biolabs offers this assay to detect the
following downstream activities to reflect the
tumor antigen specific T cell activation.
? T Cell Expansion
? Upregulation of T Cell Surface Activation
Markers
? Differentiation Into Effector Cells
? Cytokine Production or Cytotoxicity
? Induction of Apoptosis

6
Figure 1 Antitumor effects mediated by activated
CAR-T cells. (Cartellieri, et.al. 2010)
7

Highlight Features
? Senior Ph.D. scientists and extensive
experience
? One-stop service
? Superior quality
? Timely troubleshooting
? Customized assay design
8

With years of extensive experience in the field
of immunotherapy, Creative Biolabs brings our
clients a suite of relevant immune-oncology
assays for a wide range of projects. We aim to
work closely with our clients to tailor each
assay to facilitate the projects and help answer
their interesting scientific questions. Creative
Biolabs offers not only highly advanced assays
but also customized assays. Please dont hesitate
to let us know your requirement, and we will be
pleased to serve you wholeheartedly.
9
About Creative Biolabs

• With the exponentially increased demand in modern
  novel medicine, the CAR and TCR immune cell
  therapy has been one of the most promising
  approaches for target-specific cancer treatment.
  With integrated global resources in all aspects,
  Creative Biolabs provides one-stop full CAR T/NK
  Cell therapy development service, including early
  non-clinical research, process development,
  pre-clinical animal model testing, cGMP
  manufacturing, and phase I-III clinical trials.

10
Contact

• 45-1 Ramsey Road, Shirley, NY 11967, USA
• Email marketing_at_creative-biolabs.com
• www.creative-biolabs.com/car-t

11
Thank You