Title: Transcriptomics sequencing
1Transcriptomics Sequencing
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2over view
The transcriptome is the set of all RNA
molecules, including mRNA, rRNA, tRNA, and other
non coding RNA produced in one or a population of
cells. RNA Next-generation sequencing methods
have revolutionized exploration of the
transcriptome.
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3The Target of Sequencing
The transcriptomics sequencing is to sequence the
mature mRNA and ncRNA which transcribed by
affinity purified RNA polymerase II with next
generation sequencing.
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4Advantage
Advances in the sequencing workflow, from sample
preparation through data analysis, enable rapid
profiling and deep investigation of the
transcriptome.
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5The Trancsriptomics Sequencing of CD Genomics
The transcriptomics sequencing service of CD
Genomics include RNA-Seq (Transcriptome)RNA-S
eq is a powerful tool for studying the effect of
the transcriptome on information for biological,
medical, clinical and pharmaceutical research,
drug discovery and development. RNA-Seq
(Quantification)RNA-Seq (Quantification) based
on Next Generation Sequencing (NGS) is used for
transcriptome quantification and characterizing
gene expression profiling. Small RNA
SequencingSmall RNA has been to important
regulators in recent years and has significant
functions in many cellular processes including
development, cell differentiation and apoptosis,
which has been implicated in many diseases.
lncRNA Long non-coding RNA (lncRNA) is a major
hotspot in functional genomics research that
considered as non-protein-coding transcripts
longer than 200 nucleotides in length.
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6Service include
Annotate coding SNPs Discover transcript
isoforms Identify regulatory RNAs
Characterize splice junctions Determine the
relative abundance of transcripts
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7Work flow(example of lncRNA)
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8Thank you!
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