Bioactive Glass A Candidate for bone tissue regeneration

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Bioactive Glass

• A Candidate for bone tissue regeneration

Nourani M.R., PhD Visiting Research
Fellow Department of Regenerative Oral
Surgery, Unit of Translational Medicine,
Graduate School of Biomedical Sciences, Nagasaki
University  April 2015
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Bioactive glass

• The first bioactive glass (Silicon dioxide
  )SiO2(, Sodium oxide ) Na2O(, Calcium oxide
  )CaO(, and Phosphorus pentoxide )P2O5)( was
  invented by Larry Hench at the University of
  Florida in 1969.

• The original Bioactive glass has been used in
  more than a million patients to repair bone
  defects in the jaw and in orthopaedics Hench,
  2011.

• According to different reports, bioactive glass
  can be attached to soft tissue or hard tissue
  through chemical bonds(Fooladi 2013).

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A-Mechanism of bioactive glasses bioactivity
Ca2 ions are rapidly exchanged with H ions at
the reactive surface. Water (H2O) reacts to form
SiOH (silanol bond) groups at the reactive
surface. SiOH groups at the surface induce
apatite nucleation.  The apatite crystals grow
spontaneously by incorporating ions from the body
fluid. By increasing PH , this process accelerate

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B- Ionic dissolution products and osteogenesis

• Once the Apatite layer has formed, the next
  stages are that proteins adsorb to this layer,
  and cells attach, differentiate and produce bone
  matrix
• Ideally, bioactive glass implants would recruit
  osteoprogenitor cells in vivo and activate a
  bone differentiation pathway.
• In a microarray study, when human fetal
  osteoblasts were exposed to Bioglass dissolution
  products, genes associated with osteoblast
  differentiation were up-regulated.
• significant up-regulation of Runx2, Osterix(Osx),
  Dlx5, collagen I, ALP and bone sialoprotein
  (BSP) .

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Recently it is available in different form in
clinic

• Nano particle
• Granules
• Putty
• Injectable
• Solid shape
• Screw

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Number of papers published per year in the field
of bioactive glass (compiled from a
literature search in Pubmed carried out in
December 2014).
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Bone augmentation of Bioactive glass using
periosteal elevation model on rat calvaria
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• Material and Methods
• Bioglass 45s5 powder (300um size) ( Mo-Sci
  company USA, Kindly gifted by Prof. Watanabe)
• BMP , 0.2 ug/ml
• Rat Adipo- MSC (5105)
• PPP
• PRP
• Animal
• 6 weeks old Rat that classified in 8 groups
  (N-3)
• BG only ( BG 0.2gr 100 µl PPP)
• BG BMP (BG 0.2gr 50 µl ppp 50µl BMP)
• BGPRP (BG 0.2gr 100 µl PRP)
• BGCells (BG 0.2gr 50µl ppp50 µl
  MEM-a/cell)
• BGBMPPRP (BG 0.2gr 50µl PRP 50µl BMP)
• BGBMPCells (BG 0.2gr 50µl BMP50 µl
  MEM-a/cell)
• BGPRPCells (BG 0.2gr 50µl PRP50 µl
  MEM-a/cell)
• BGBMPPRPCells (BG 0.2gr 50µl BMP50µl
  PRP50 µl MEM-a/cell)

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Sample preparation
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Percentage