Creative biogene-The Extended Applications of RNAi

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The Extended Applications of RNAi
Presented by Creative Biogene
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RNA interference

• RNA interference, known as RNAi, has expanded
  dramatically in a short-term period since its
  first discovery in 1990s. Several types of small
  silencing RNAs have now been discovered,
  including small interfering RNAs (siRNAs),
  microRNAs (miRNAs) and PIWI-interacting RNAs
  (piRNAs). Small RNAs are involved in regulating
  gene expression in many ways, and RNAi is being
  harnessed by scientists for laboratory research
  and for the development of new therapies for
  disease.

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The most interesting aspects of RNAi are the
following

• dsRNA, rather than single-stranded antisense RNA,
  is the interfering agent
• it is highly specific
• it is remarkably potent (only a few dsRNA
  molecules per cell are required for effective
  interference)
• the interfering activity (and presumably the
  dsRNA) can cause interference in cells and
  tissues far removed from the site of introduction

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All about RNAi

• Biological functions of RNAi
• Immunity
• Downregulation of genes
• Upregulation of genes
• General Applications of RNAi
• Gene knockdown
• Functional genomics
• Therapeutic gene modulation
• Antiviral
• Cancer
• Safety
• Applications of RNAi in biotechnology
• Foods (genetically engineer plants) produce
  lower levels of natural plant toxins
• Insecticide 
• Transgenic plants
• Genome-scale screening

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Custom RNAi services provided by Creative
Biogene 

• siRNA, shRNA and miRNA design and synthesis
• For this, we need customer provides target gene
  name, sequence or GeneBank ID and so on and we
  will do the others.
• Choice of shRNA and miRNA vector.
• siRNA, shRNA and miRNA transfection optimization
  service.
• Test of inhibitory effect the mRNA expression is
  detected by RT- PCR the protein expression is
  detected by Western Blot.
• Provision of experimental reports RNAi sequence,
  RT-PCR test report, Western Blot picture and
  so on.
• Custom preparation of shRNA and miRNA expressing
  lentiviral particles, determination of the
  virus titer and establishment of stable cell
  lines.

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Other services provided by Creative Biogene

•  Custom Viral Service  RNAi Service   Gene
  Service  Aptamer Service  Plasmid DNA
  Production Service  Stable Cell Line
  Generation   Oligonucleotide Synthesis Service

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Contact

• USA45-1 Ramsey Road, Shirley, NY 11967, USATel
  1-631-626-9181Emailinfo_at_creative-biogene.com
• United Kingdom St John's Innovation Centre,
  Cowley Road, Cambridge CB4 0WS, UK Tel
  44-207-048-3343 Emailinfo_at_creative-biogene.com
• Web http//www.creative-biogene.com/Services/RNAi
  -Service.html