Plasmid DNA — an indispensable tool for molecular biology

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Plasmid DNAAn Indispensable Tool for Molecular
Biology by Creative Biogene
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Definition

• A plasmid is a small DNA molecule within a cell
  that is physically separated from a chromosomal
  DNA and can replicate independently.
• Mostly found in bacteria (presents to be as a
  small, circular, double-stranded DNA moleculeas
  the picture shows), also found in archaea and euka
  ryotic organisms.

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Natural and Artificial Plasmids

• In nature, plasmids often carry genes that is
  essential for the survival of an organism.
  Therefore people generate artificial plasmids
  that are widely used as vectors to drive the
  replication of recombinant DNA sequences within
  host organisms. They can be used for
• Cloning
• Protein production
• Gene therapy
• Disease models
• Or we could say that plasmids serve as cell
  transfection, support DNA vaccine development,
  antibody production, and other preclinical and
  clinical research projects.

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Advantages

• In addition to reasons above, factors that make
  plasmids either more useful or easier to work
  with include
• 1. They are small. If they are small, they
  are easier to isolate (you get more), handle
  (less shearing), and transform.
• 2. They have multiple restriction enzyme
  sites. More sites give you greater flexibility in
  cloning, perhaps even allowing for directional
  cloning.
• 3. They have multiple ORIs. It is important to
  note that two genes must have different ORIs if
  they are going to be inserted in the same plasmid.

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Plasmid DNA Purification

• Plasmid DNA purification is an very important
  technique for various molecular biology and
  biochemistry labs. They can be used for a variety
  of applications such as, research, transfection,
  preclinical, bacterial transformation, clinical,
  restriction endonuclease digestion, diagnostic,
  PCR, sequencing, and in vitro transcription, etc.

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Commercial SupplierCreative Biogene

• Creative Biogene has been perfecting plasmid DNA
  production for many years. We offer a wide range
  of plasmid preparation services for many
  applications, including research, preclinical,
  clinical, and diagnostic applications. The
  plasmid DNA isolation service will be suitable
  for transfection and animal studies to both
  biotechnology researchers and pharmaceutical
  companies. Our goal is to provide you with the
  most affordable, high-quality plasmid DNA
  manufacturing services that ensure your
  satisfaction in a timely and professional manner.

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Features of Creative Biogenes Plasmid DNA
Production Service

• Construct your plasmid according to your
  requirements. Produce plasmids for applications
  both in vitro and in vivo. Robust manufacturing
  protocols. Flexible scale up to gram level.
  High standard quality control. Short
  turn-around time.

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Other services provided by Creative Biogene

•  Custom Viral Service  RNAi
  Service   Gene Service  Aptamer
  Service  Plasmid DNA Production
  Service  Stable Cell Line Generation   Oligonu
  cleotide Synthesis Service

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Contact

• USA45-1 Ramsey Road, Shirley, NY 11967, USATel
  1-631-626-9181Emailinfo_at_creative-biogene.com
• United Kingdom St John's Innovation Centre,
  Cowley Road, Cambridge CB4 0WS, UK Tel
  44-207-048-3343 Emailinfo_at_creative-biogene.com
• Web http//www.creative-biogene.com/Services/Plas
  mid-DNA-Production-Service.html