Introducing the DSQII

1

• Introducing the DSQII
• Terry Jeffers

2
Agenda

• Update on Thermos Environmental Initiatives
• Discussion on new approaches to the challenges of
  8270
• Introducing Thermo Electrons Productivity Kits
• EnviroLab Forms 2.0 High Throughput
  Quatification
• New application note Rapid Analysis of 8270

3
Thermo Electron Environmental Initiative

• Focus on Environmental Laboratory needs
• Requirement for Productivity
• Need for good work flow in Generating, Reviewing
  and Reporting data
• Benefit from guides for operation, maintenance
  and troubleshooting specific Methodologies

4
Thermo Electron Environmental Initiative
Product Sales Specialist
Environmental Demo Chemist

Environmental Seed Chemist
New hardware DSQ II
Productivity Kits
8270 and 525
8260 and 524
5
Pitt Falls for Method 8270

• Those little things that stop everything

6
Activity in the GC/MS system

• Activity
• These sites can be everywhere
• Injector, column, source
• SPCC compounds are used to show good system
• Nitrophenols are some of the best compounds to
  find active sites
• Minimizing Effects of Activity
• Deactivated liners
• Good column that shows no active sites
• High flow rates to reduce the residence time of
  any active compound.
• Balance temperature zones to reduce activity and
  thermal break down.

7
Chromatography

• How important is it really?

8
GC and Autosampler

• Separations accomplished with slow chromatography
• Common GC methods for 8270
• Fast injection
• 250 - 300 0 C injector temperature
• 1 mL/min flow rate
• 30 m x 0.25 mm ID column
• Oven ramps between 5 0 and 10 0 C/min from 35 0
  to 300 0 C
• Separations accomplished with fast chromatography
• Alternate GC method for 8270
• Hot needle injection
• 250 0 C injector temperature
• 3 mL/min flow rate
• 30 m x 0.25 mm ID x 0.5 µm column
• Oven stepped ramps to provide the critical pair
  separations.

9
Hot Needle Injection Run at Lower Inlet
Temperature Fewer Replacements of Liner
10
Increase the Mobile Phase 3 mL/min vs 1mL/min

• Increase capacity of stationary
• No fronting of peak shape for 160 ng/uL PNA
• Decrease the irreversible absorption of active
  species
• Pentachlorophenol lt 10RSD
• (5-160 ng/uL)
• 0.1 ng/uL MDL
• replicates at 1 ng/uL 4.7RSD
• Minimal breakdown for endrin or 4,4-DDT
• Provide Gaussian shape peaks for the most
  volatile
• No solvent skewing of NDMA and pyridine
• Shorter run times 17 minutes

11
Effects of increased flow rates

• Why increase the flow rate to 3.0 mL/min
• Learned from applications developed for the
  analysis of explosives.
• Decrease the residence time of the compounds in
  potential active areas.
• Decreases the run time without higher
  temperatures that could potentially damage
  thermally labile compounds.
• Do not need to expose the column to higher
  temperatures that will damage it.
• Provides better separations and narrower peak
  widths.
• This is balanced with a 201 split flow ratio.

12
Injection Choice of Liner
13
PNAs ( 5 ng and 160 ng)
14
NDMA and Pyridine
Split
Splitless
15
Two Methods Splitless and Split
16
TRACE Ultra DSQ II GC/MS
17
Source materials and geometry

• New DuraBriteTM Source Open Geometry
• Passive Collector
• Extends Dynamic Range
• Improves Robustness
• Optional Closed EI Ion Volume
• Constructed of new proprietary material for
  enhanced inertness
• Protects Lens 1 from ion burn

18
New DynaMax XR Ion Detection System

• New Linear/Log Hybrid Electrometer
• Extends output current to 70µA
• Provides dynamic range to gt106
• No loss of response or precision at the lower
• sample/analyte levels

• SGE ETP AF635 Electron Multiplier
• Detector linear range
• output current gt30µA
• Simple snap out replacement of
• multiplier assembly

19
Expected Results

• Results I can get, you can get them too.

20
Calibration Curve

• First page of the calibration curve report
• All SPCC and CCC requirements met
• Average of all RSD calibration curves of 7.46

21
SPCCs (5ng to 160 ng min gt0.05)
22
CCCs lt 30 RSD for Initial Calibration Curve
23
Quantitated Data File

• First page of the Quantitation Report
• This shows the quantitation of the 20 ng/uL
  sample for the calibration curve
• Meets the requirements of the internal standards
  and surrogates
• Meets all ion ratio requirements
• No manual integrations were required

24
Robustness Split (2gr sediment/2mL methylene
chloride 25 uL diesel fuel)
25
How can we prove it

• Productivity Solutions

26
Productivity Solutions

• Integrating the DSQ II with Software, Methods,
  and Consumables to Get Productive, Quickly

27
Productivity Solutions

• Provide all of the necessary material to run the
  method
• Proven Thermo Column
• Deactivated Liners
• Standards
• Snap and shoot prediluted for entire method
  validation
• All other consumables for about 2 weeks
• Provide all of the software
• EnviroLab Forms 2.0
• Interactive Reference CD
• All of the methods, sequences,
• Validation data used to generate all of what you
  see here and in the manuals
• Two Boxes full of materials, manuals, and CDs.

28
Productivity Kit

• SOP manual for method
• How to Guide
• Quick Start Guide
• Provide consumables for running method
• Standards, liners, septa, syringes, column,
• Instrument method, processing method, reporting
  package
• CD deliverable
• SOP
• Data from method validation
• Instrument method
• Processing method
• Reporting software with templates

29
Pieces of the Productivity Kit
30
Quick Start Guide
31
8270C/D Productivity Solution

• Customers system can be taken from installation
  sign off to method IDLs in as little as 2 days!
• 8270 is a value added product which will sell for
  3000 US
• Add on service provided by Thermo FSEs
• US Field Service Engineers are being trained on
  the 8270 PS
• Kits serve as a great tool for training aides for
  the lab

32
Productivity Solutions are a Win-Win

• Good for customers
• Improves overall experience
• Increases satisfaction
• Eases transition to Thermo GC/MS
• Gives optimal performance for the DSQ II
• Good for Thermo
• Easy to support these solutions

Helps the customer and support by establishing a
known set of criteria to achieve success. Address
the new lab environment less highly skilled users
with higher throughput requirements
33
Redefining the Way We Look at GC/MS
Productivity Solutions target routine GC/MS
applications to offer value to the customer
34
EnviroLab Forms v 2.0High Throughput
Quantification
35
EnviroLab Forms v 2.0

• An entirely new data processing and reporting
  system running as a layer over Xcalibur 1.4
• While targeted towards environmental laboratories
  and applications, it has been shown to be
  valuable for any laboratory performing
  high-throughput quantification
• Adds a significant complement of capabilities
  that Xcalibur
• Ion ratio calculations
• Peak integration
• Reporting
• Integrated data review and reporting
• No more batch reprocessing to re-generate reports
  of edited results
• Fully automated while still allowing
  sophisticated manual review

36
Data Review
37
Report View
38
EnviroLab Forms v 2.0

• Brand new workflow oriented user interface
• Substantially improved ion ratio review and
  editing support
• Comprehensive set of textual and graphical
  reports
• Fully automated, fully manual or any combination
  in between
• Can be used as a high-throughput quantification
  engine for any application
• Simply turn off or dont use those options which
  arent relevant for a given user

39
Conclusion

• The DSQ II system provides updates to hardware
  and software that make it easy to use
• The Productivity Solution for method 8270
  provides a method that has been validated across
  multiple DSQ II systems.
• All of this allows a new operator to be
  productive immediately
• The proven changes to the method allow an
  increased amount of productivity and throughput
  for the analyst and the laboratory.

40

• Questions?

Thank You