Enzymatic Determination of Creatine Kinase CK Activity

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Enzymatic Determination of Creatine Kinase (CK)
Activity
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So what does that mean?

• CK is an enzyme that everyone has in their
  bodies.
• It is mainly located in the heart skeletal
  muscles.
• Damage to these causes CK to be released into the
  bloodstream.
• Therefore, if you test the blood find a
  heightened level of CK, it can be determined that
  the body has received damage.

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However

• There is more than one type of CK therefore,
  more tests are required.
• CK2 comes from the heart while CK3 comes from the
  muscles.

-More on that from Justin Kendra.
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How its done

• First, you take the CK Reagent (2.4ml this
  contains everything needed to react with the CK
  in the samples).
• Then, you add your sample (0.1ml) mix with the
  vortex-genie.
• Next, you put it into the prepared
  spectrophotometer read the absorbance reading
  provided by the machine at given times.

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What is a spectrophotometer, why did we use one?

• A spectrophotometer is a device that shines light
  into a sample measures how much light was
  absorbed by the sample.
• We used one because the products of the CK CK
  Reagent reaction are light absorbent.
• Therefore, the samples with more CK will have
  more products which will absorb more light.

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What we learned

• How to properly use lab equipment including
  spectrophotometers, pipettes, pipetters,
  capillaries, etc.
• What CK is how it relates to our heart
• How to measure CK
• How not to get CK Reagent on ourselves