Barley, Beer, and Associations with Wolves

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Barley, Beer, and Associations with Wolves CIHEAM
International Masters in Plant Breeding May 4,
2009 Lecture 1
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Barley
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Beer
Reinheitsgebot 1516
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Association (mapping)
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Dr. Bob Wolfe
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Wolfe R.I. and J.D. Franckowiak. 1991. Multiple
dominant and recessive genetic marker stocks in
spring barley. Barley Genetics Newsletter.
20117121
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Doubled Haploid Production
X
Hordeum bulbosum 2N 2X 14
Hordeum vulgare 2N 2X 14
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Interspecific Cross
Genotype Vv Wst wst
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2. Spontaneous elimination of H. bulbosum
chromosomes.
1. Fertilization triggers embryo development.
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3. Development of haploid H. vulgare embryos
representing a random sample of gametes.
N 7
4. Rescue and culture of haploid embryos.
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5. Double chromosome complement with colchicine.
2N 2x 14
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6. Progeny are a set of homozygous genotypes
representing a random sample of F2 gametes.
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OWB-D X OWB-R
F1
94 doubled haploids
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http//barleyworld.org/oregonwolfe.php

• An immortal reference population
• Share homozygous seed
• Continually add data
• Build and share research and instruction tools

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Chutimanitsakun et al. In preparation. 2383 loci
RAD markers (Floragenex)
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1H 2H
3H 4H
5H 6H 7H
MFT
VRN-H3
Ppd-H1
Ppd-H2
VRN-H2
blp
VRN-H1
wst
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P. Szucs, V. Blake, P.R. Bhat, S. Chao, T.J.
Close, A. Cuesta-Marcos, G.J. Muehlbauer, L.
Ramsay, R. Waugh, and P. M. Hayes 2009. An
integrated resource for barley linkage map and
malting quality QTL alignment. The Plant Genome.
In press.
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2-row vs. 6-row
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Extent of LD in the CAP Core (all)
r2
Genetic Distance (cM) Chromosome 2H
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Accounting for structure
Markers
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Winter vs. Spring
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Winter vs. spring

• Winter cereals
• Better water use efficiency
• Higher yield
• Rotational advantages

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Hulled vs. Hull-less
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Food Barley

• Health benefits
• High fiber
• LDL cholesterol reduction
• Type II diabetes management
• Reduce Obesity
• Increase Satiety

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Cistue et al. In preparation 100 new OWBs RAD
map