Anotation Process

1
Scenario 3
Anotation Process
What follows is a simulation of the process of
annotating, using the proposed graphical
interface. The interface does not yet exist. As
you go through the simulation please consider
what capabilities you would want to serve your
research and annotation interests. A narrative to
help you go through the simulation appears in a
red-bordered box, such as the one below.
To begin1. Click on Slide Show, (on the upper
toolbar)2. Click View Show3. Click Continue
button
Continue
2
Scenario 3
Anotation Process
You find yourself annotating a set of genes that
includes all1290 from Anabaena PCC 7120. You
want to assess the validity of the automated
annotation, particularly the assertion of
function and start codon. If there are any
problems, you want to fix them.
Continue
3
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History
Anabaena PCC 7120 all1290
Replicon Chromosome Coordinates 1533026 (stop)
lt- 1533628 (start-GTG) System Length 200
amino acids Strand Complementary Function PvuII
DNA methyltransferase System Cyanobacterial
orthologs none
Anab7120
Proteus vulgaris
Salmonella paratyphi
Streptomyces spectabilis
Evidently no human has touched this annotation
(System is given as the only source). Is the
functional annotation sound? Mouse over to the
blue motif bar above the graphical representation
of all1290. In real life, information will pop
up when you mouse to an informative position. In
this simulation youll also need to click.
4
Options
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History
Anabaena PCC 7120 all1290
Replicon Chromosome Coordinates 1533026 (stop)
lt- 1533628 (start-GTG) System Length 200
amino acids Strand Complementary Function PvuII
DNA methyltransferase System Cyanobacterial
orthologs none
N6,N4 DNA methyltransferase (PF01515) 2e-18
Anab7120
Proteus vulgaris
Salmonella paratyphi
Streptomyces spectabilis
Pfam found a motif consistent with the annotation
with a good expectation value. Curiously, the
motif extends beyond the start of the gene. Mouse
away from the bar. In real life, information
will go away when you mouse away from an
informative position. In this simulation youll
also need to click anywhere..
5
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Annotate
History
Anabaena PCC 7120 all1290
Replicon Chromosome Coordinates 1533026 (stop)
lt- 1533628 (start-GTG) System Length 200
amino acids Strand Complementary Function PvuII
DNA methyltransferase System Cyanobacterial
orthologs none
PvuIIM DNA methyltransferase (NP_072082) 8e-64
Anab7120
Proteus vulgaris
Salmonella paratyphi
Streptomyces spectabilis
Mouse over to the first aligned gene, from
Proteus vulgaris. Again, good support, and again,
the gene extends beyond the annotated start codon
of the Anabaena orf. Remember to click to view
the popup and click to make it disappear.
6
Options
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Anabaena PCC 7120 all1290
Replicon Chromosome Coordinates 1533026 (stop)
lt- 1533628 (start-GTG) Predicted Length
200 amino acids Strand Complementary Function
PvuII DNA methyltransferase Predicted Cyanobacteri
al orthologs none
Anab7120
Proteus vulgaris
Salmonella paratyphi
Streptomyces spectabilis
You begin to suspect the annotated start codon.
To visualize alternatives, click on the Options
button, then click on Show alternate starts.
Options
7
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Annotate
History
Anabaena PCC 7120 all1290
Replicon Chromosome Coordinates 1533026 (stop)
lt- 1533628 (start-GTG) System Length 200
amino acids Strand Complementary Function PvuII
DNA methyltransferase System Cyanobacterial
orthologs none
1533628 and upstreamCTTCCGGGAAATCCTGTG
1533931 and upstreamTAACTTAAATGTCGCTTG
Anab7120
Proteus vulgaris
Salmonella paratyphi
Streptomyces spectabilis
Mouse over to the annotated start site and you
see the sequence preceding it a plausible
ribosome binding site. Mouse over to the most
distant start site. Not as good. Click to show,
click to disappear
8
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Annotate
History
Anabaena PCC 7120 all1290
Replicon Chromosome Coordinates 1533026 (stop)
lt- 1533628 (start-GTG) System Length 200
amino acids Strand Complementary Function PvuII
DNA methyltransferase System Cyanobacterial
orthologs none
Anab7120
Proteus vulgaris
Salmonella paratyphi
Streptomyces spectabilis
Nonetheless, the position of the distant site
relative to the other sequences makes it a
reasonable candidate. Click on it to
provisionally change the start site.
9
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Annotate
History
Anabaena PCC 7120 all1290
Replicon Chromosome Coordinates 1533026 (stop)
lt- 1533628 (start-GTG) System Length 200
amino acids Strand Complementary Function PvuII
DNA methyltransferase System Cyanobacterial
orthologs none
Anab7120
Proteus vulgaris
Salmonella paratyphi
Streptomyces spectabilis
Streptomyces netropsis
Much better! More similarity and more sequences
that are similar (and better E-values, too, if
you were to look). However, it is always a good
practice to check the alignment before accepting
the conclusions of a similarity search, so click
on button. and then on View.
Neisseria meningitidis
More
Options
10
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History
Options
Anabaena PCC 7120 all1290
View Show all Show hits lt1e-6 Show neighbors
/- 1 Show motifs Show alternate starts Show
annotated features Show predicted features Show
transposon ends Show tandem repeats Show inverted
repeats Show dispersed repeats Show private set
GraphicGenome Alignment with homologs Alignment
with motif
View
Replicon Chromosome Coordinates 1533026 (stop)
lt- 1533628 (start-GTG) System Length 200
amino acids Strand Complementary Function PvuII
DNA methyltransferase System Cyanobacterial
orthologs none
Alignment with motif
Anab7120
Proteus vulgaris
Salmonella paratyphi
Streptomyces spectabilis
Streptomyces netropsis
Click on Alignment with motif to see how All1120
compares to other protein that make up the Pfam
motif represented by the motif bar.
Neisseria meningitidis
More
11
Conserved Domain Database
CD
Pfam01555.5, N6_N4_Mtase
PSSM-Id
8054
Source
PfamUS, PfamUK
Description
DNA methylase. Members of this family are DNA
methylases. The family contains both N-4
cytosine-specific DNA methylases and N-6
Adenine-specific DNA methylases.
Taxa
root
Status
Created
Alignment from source
13-Jun-2002
Aligned
52 rows
PSSM
199 columns
Representative
Consensus
Click here for CDART summary of Proteins
containing pfam01555
Proteins
with  Cn3D using  Virtual Bonds
(To display structure, download Cn3D)
View 3D Structure
Subset Rows
up to 10
of the most diverse members
10 20
30 40 50 60
............................
.................... All1290
78 KRVLTENGSFILDLGgaylpgnp----VRSIYQYELLVKLC
KEVGFFL----------- 122 consensus
1 ARRVLKPDGSIFIFGG------------NKSQKNIFRFIDILIEKGF
KF----------- 37 Pvuii Dna Methyltransfer 77
DGSFVVDFGGAYMKGVp-----------ARSIYNFRVLIRMIDEVGFFL-
---------- 114 Modification methylase S 119
PRTGWKRNKQLSLVP--------------------FRVALALQDDGWWI-
---------- 147 BglI modification methyl 92
FQAVNLKKHRVSVTREd----------iLNALKLEPELTKYQLAKKFNCs
---------e 132 adenine specific DNA met 174
ARDLLKEDGVIFISIDdnecan-lkilcDEIFGEDNFVGDFIRKTKSTTn
dakiglnyqh 232 adenine specific DNA met 131
ILRTLLPIYARALKDLmrt------iknVFKQKGQAQAQLVLKEQIKELs
---------q 175 Modification methylase M 133
IIDPFMGSGTTLVQCMem--------giNSIGIDISPFNCLIAEVKLQKy
di------qk 178 Modification methylase M 178
LSRCNLKDGDYILDPFagtgttgavvkkMKYQLYPKDLNVILIEKGKKF-
---------- 226 Modification methylase B 134
SHIAFEPKSFIFEYNLeeldraykeiyeISPTKIEQKFNHLSITEGAFPe
ete----ndl 189 gi_3257411 123
ANTYAMPSELILKGFP-------------KVKDVVLLFVDDKVLVGETVr
---------- 159
This is output from NCBI showing the alignment
that went into making Pfam motif 01555... but it
was intercepted by the interface in order to
incorporate the sequence of All1290 for
comparison. Click on toget to
the best part of the alignment.
More
Back to Annotation
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12
Conserved Domain Database
310
320 330 340 350 360
....................
............................ All1290
228 rrcKAAGVKPHPARFPQGFAEFFIKFLTD----
---------------EGDLVLDPFAGS 268 consensus
131 ---NLKGKKKHPTQKPEALLERIILESSN-------
------------EGDLVLDPFAGS 168 Pvuii Dna
Methyltransfer 223 ancKLMGIKAHPARFPAKLPEFFIRMLTE-
------------------PDDLVVDIFGGS 263 Modification
methylase S 339 sipTAPSGANHFAVMPRKLAHFALKATLP----
---------------MNGSCLDPFMGS 379 BglI
modification methyl 238 ---SVRKNDDHEAKFPLLLPQRLIK
LLTQ-------------------KGDTVLDCFMGS 275 adenine
specific DNA met 367 ellNLGMGEYFTYPKGVEFMKKIILHST
Tp-----------------nEGDIILDFFAGS 409 adenine
specific DNA met 279 ----LGLKGLFKTPKPVALIKYLLLCST
P-------------------KDSIILDFFAGS 315
Modification methylase M 329 vfdPYYCYKHKKICRPVQT
ILRHLEEYTNdvisri-------eefskiRKDAYYLIINGD 381
Modification methylase M 464 icyNCQKEIYGKNSLPILK
ESKEIIESLEsvengeyqvgenikpqyqkRCKESKSKFAGM 523
Modification methylase B 319 tspPYCNRYDYTRTYALEL
AYLGVDEKKIrelrqa-------qlsctvENKSKLKQLKDY 371
gi_3257411 255 ---KVCDVRRLRKCFPRTR
FDAIITEPYM-------------------GKPLKYKPSRGE 292
370 380
390 400 410
................................
............. All1290 269
NTTGFVAETW-----------QRRWIAVEINQDYVLGS------------
--RYRF 299 consensus 169
GTTAAAAKEL-----------GRKFIGIEINEEYVKLA------------
--KKRL 199 Pvuii Dna Methyltransfer 264
NTTGLVAERE-----------SRKWISFEMKPEYVAAS------------
--AFRF 294 Modification methylase S 380
GTTGRVVREL-----------GGRFVGVDVNEHYMTDY------------
--LVES 410 BglI modification methyl 276
GTTAVAALSE-----------SRNFIGIEKEPKYIQLS------------
--NKNV 306 adenine specific DNA met 410
GTTVHAVMELnae-----dkgNREFILVQIDEEIKEDEsay--------d
fcKKEL 452 adenine specific DNA met 316
GTTAQAVIEV-----------NRDYCLNWSFYLCQKEE------------
--KIKN 346 Modification methylase M 382
SRTVDIEEELkkhpnfyelykNKKIDGIFTSPPYLGQIdyheqhayayel
fdIPRL 437 Modification methylase M 524
ERMNWGASPG-----------ARKTIIGDSFSKMRLYR------------
--LDQP 554 Modification methylase B 372
YHSLFLESRYae--------iERLVTGNEVLNEINYAL------------
--RKRW 405 gi_3257411 293
AIKIAKGLDR------------LYYQAFESFADVLKRGs-----------
--IIVF 323
Note that All1290 matches the alignment in almost
all of the most informative positions (in red),
those presumably related to common function.
Click on
Back to Annotation
Back to Annotation
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13
Main Menu
Options
Annotate
History
Anabaena PCC 7120 all1290
Replicon Chromosome Coordinates 1533026 (stop)
lt- 1533628 (start-GTG) System Length 200
amino acids Strand Complementary Function PvuII
DNA methyltransferase System Cyanobacterial
orthologs none
Anab7120
Proteus vulgaris
Salmonella paratyphi
Streptomyces spectabilis
Streptomyces netropsis
Since the motif does not extend to the beginning
of the protein, it might be a good idea to check
alignment with the full length of protein
identified by the similarity search. Click on
button then on View, and finally on
Alignment with homologs.
Neisseria meningitidis
More
Options
14
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History
Anabaena PCC 7120 all1290
NmeSIM (Neisseria) 3 ---TISSIMSLEN----------
-----FQLIKTQLGVPSYEND--RVAIYQGDCIQLMD ScaIM
(Streptomyces) 6 FGYVIQSSAALWNRLSTFSQRGKALDTRL
ADIKKALGKPYYETS--DVLLYHGDSLELLK All1290
(Anabaena) 1 LNSQQTTTIKLTN------------------
-----FIPSYTQQ--HGAVYLGDCLEIIK PvuIIM (Proteus)
6 LQTMSSNDMLNFG-----------------------KKPAYTT
S--NGSMYIGDSLELLE SptAIM (Salmonella) 1
--------MMFDD-----------------------KKPAYKTD--LGAM
YIADSLEMLE SpcM (Streptomyces) 1
----MTESTTAAG-----------------------DRRALGAE--HGSY
HWGDSLALLE SpcG (Streptomyces) 1
----MTELT-PTE-----------------------DRCPLEAE--HGSY
HWGNSLELMK BglIIM (Bacillus) 5
QYKQIKLHLGMED---D-----------NEDLPNHIPSSFPKQH--LNKI
YNGDTMNMLL MJ1498 (Methanococcus) 1
------------------------------------------ME--INKI
YCMDCLEGMK CfrBIM (Citrobacter) 66
VINSKTSTIDYKKSSKSPFLIKKAMEVVHTPKKVKKEKKIISKSPLNNML
LQGNCAETLK BamHIM (Bacillus) 68
KILENKNSVKLNEFN-----------------SIQEIKPYFYTD--LGKL
YNGDCLELFK
NmeSIM (Neisseria) 43 KISKGSFQMTITSPPYNIGKEYE
S----ILDLEHYLEWCERWMQSIHHLTEDSGCFWLNL ScaIM
(Streptomyces) 64 SMPQQIFDLTVTSPPYNIGKEYEG----V
LSIEEYISWCETWMSRVHRATSAGGAFWLNV All1290
(Anabaena) 36 SIPDNSVNLILTSPPFALTRKKEYG---NES
AEKYIEWFLPFADEFKRVLTENGSFILDL PvuIIM (Proteus)
41 SFPDESISLVMTSPPFALQRKKEYG---NLEQHEYVDWFLSFA
KVVNKKLKPDGSFVVDF SptAIM (Salmonella) 28
SMPDNSLNLVMTSPPFALQRKKEYG---NHDQEQYIDWFLKFGELVFKKL
KDDGSFVVDF SpcM (Streptomyces) 32
GVPDGSVRAVVCSPPFEGPQLIADE---DRAGACSSDWLMPFFGQFERVL
RPDGCVAFEL SpcG (Streptomyces) 32
GIPDASVDAVVCSPPFEGDALISDE---GRRGDAFVAWIEPFFRQFQRVL
RPAGCVAFEL BglIIM (Bacillus) 49
DIPDNSVDLVVTSPPYNINKFKND----RRPLEEYLKWQTEIIEQCHRVL
KPSGSIFWQV MJ1498 (Methanococcus) 17
QLKDKTVDVVVTSPPYNIGIKYNKYSD-NLSREDYLNWIEEVVKEIKRVL
KDDGSFFINV CfrBIM (Citrobacter) 126
KLPDESVNLVFTSPPYYNAKPEYS--E-YHTYDEYLSLLRSVIKECHRVL
SEGRFFVINV BamHIM (Bacillus) 68
QVPDENVDTIFADPPFNLDKEYDEGVTDKNSFSGYLDWYYKWIDECIRVL
KPGGSLFIYN
To one knowledgeable on DNA methyltransferases,
the identity of All1290 at positions 48-51 (SPPF)
is particularly striking, as that is part of the
AdoMet binding site and one of the signatures of
N4-cytosine methyltransferases. Press
.
Continue
Continue
15
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History
Anabaena PCC 7120 all1290
NmeSIM (Neisseria) 3 ---TISSIMSLEN----------
-----FQLIKTQLGVPSYEND--RVAIYQGDCIQLMD ScaIM
(Streptomyces) 6 FGYVIQSSAALWNRLSTFSQRGKALDTRL
ADIKKALGKPYYETS--DVLLYHGDSLELLK All1290
(Anabaena) 1 LNSQQTTTIKLTN------------------
-----FIPSYTQQ--HGAVYLGDCLEIIK PvuIIM (Proteus)
6 LQTMSSNDMLNFG-----------------------KKPAYTT
S--NGSMYIGDSLELLE SptAIM (Salmonella) 1
--------MMFDD-----------------------KKPAYKTD--LGAM
YIADSLEMLE SpcM (Streptomyces) 1
----MTESTTAAG-----------------------DRRALGAE--HGSY
HWGDSLALLE SpcG (Streptomyces) 1
----MTELT-PTE-----------------------DRCPLEAE--HGSY
HWGNSLELMK BglIIM (Bacillus) 5
QYKQIKLHLGMED---D-----------NEDLPNHIPSSFPKQH--LNKI
YNGDTMNMLL MJ1498 (Methanococcus) 1
------------------------------------------ME--INKI
YCMDCLEGMK CfrBIM (Citrobacter) 66
VINSKTSTIDYKKSSKSPFLIKKAMEVVHTPKKVKKEKKIISKSPLNNML
LQGNCAETLK BamHIM (Bacillus) 68
KILENKNSVKLNEFN-----------------SIQEIKPYFYTD--LGKL
YNGDCLELFK
NmeSIM (Neisseria) 43 KISKGSFQMTITSPPYNIGKEYE
S----ILDLEHYLEWCERWMQSIHHLTEDSGCFWLNL ScaIM
(Streptomyces) 64 SMPQQIFDLTVTSPPYNIGKEYEG----V
LSIEEYISWCETWMSRVHRATSAGGAFWLNV All1290
(Anabaena) 36 SIPDNSVNLILTSPPFALTRKKEYG---NES
AEKYIEWFLPFADEFKRVLTENGSFILDL PvuIIM (Proteus)
41 SFPDESISLVMTSPPFALQRKKEYG---NLEQHEYVDWFLSFA
KVVNKKLKPDGSFVVDF SptAIM (Salmonella) 28
SMPDNSLNLVMTSPPFALQRKKEYG---NHDQEQYIDWFLKFGELVFKKL
KDDGSFVVDF SpcM (Streptomyces) 32
GVPDGSVRAVVCSPPFEGPQLIADE---DRAGACSSDWLMPFFGQFERVL
RPDGCVAFEL SpcG (Streptomyces) 32
GIPDASVDAVVCSPPFEGDALISDE---GRRGDAFVAWIEPFFRQFQRVL
RPAGCVAFEL BglIIM (Bacillus) 49
DIPDNSVDLVVTSPPYNINKFKND----RRPLEEYLKWQTEIIEQCHRVL
KPSGSIFWQV MJ1498 (Methanococcus) 17
QLKDKTVDVVVTSPPYNIGIKYNKYSD-NLSREDYLNWIEEVVKEIKRVL
KDDGSFFINV CfrBIM (Citrobacter) 126
KLPDESVNLVFTSPPYYNAKPEYS--E-YHTYDEYLSLLRSVIKECHRVL
SEGRFFVINV BamHIM (Bacillus) 68
QVPDENVDTIFADPPFNLDKEYDEGVTDKNSFSGYLDWYYKWIDECIRVL
KPGGSLFIYN
The substantial agreement between All1290,
PvuIIM, and SptAIM turns out to extend through
the DNA recognition domain. Thats good enough
for me! Lets start making this public by
clicking .
Annotate
16
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Options
Annotate
History
Anabaena PCC 7120 all1290
Replicon Chromosome Coordinates 1533026 (stop)
lt- 1533628 (start-GTG) System Length 200
amino acids Strand Complementary Function PvuII
DNA methyltransferase System Cyanobacterial
orthologs none
Anab7120
Proteus vulgaris
Salmonella paratyphi
Streptomyces spectabilis
Streptomyces netropsis
That click gets you back to the annotation page.
Lets begin by changing the start site. Click
Start site..
Neisseria meningitidis
More
17
Main Menu
Options
Annotate
History
Anabaena PCC 7120 all1290
Replicon Chromosome Coordinates 1533026 (stop)
lt- 1533628 (start-GTG) System Length 200
amino acids Strand Complementary Function PvuII
DNA methyltransferase System Cyanobacterial
orthologs none
1533628
Anab7120
Proteus vulgaris
Salmonella paratyphi
Streptomyces spectabilis
Streptomyces netropsis
You could type in the new coordinate, but its
easier just to click on the new start site.
Neisseria meningitidis
More
18
Main Menu
Options
Annotate
History
Anabaena PCC 7120 all1290
Replicon Chromosome Coordinates 1533026 (stop)
lt- 1533628 (start-TTG) Predicted Length
301 amino acids Strand Complementary Function
PvuII DNA methyltransferase Predicted Cyanobacteri
al orthologs none
1533931
Justification IC Inferred by curatorIDA
Inferred by direct assay IEP Inferred from
expression pattern IGI Inferred from genetic
interaction IMP Inferred from mutant
phenotype IPI Inferred from physical
interaction TAS Traceable author statement NAS
Nontraceable author statement ND No biological
data available
IC Inferred by curator
IC Inferred by curatorUse this when your
assertion is based on your own judgement of
sequence similarity or other information, when
there is no direct experimental data.
Anab7120
Proteus vulgaris
Salmonella paratyphi
Streptomyces spectabilis
Streptomyces netropsis
Every assertion must be accompanied by a
justification. The codes are standard from the
Gene Ontology Consortium and facilitate
searching. Mouse over to IC to find out what its
used for.
Neisseria meningitidis
More
19
Main Menu
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Annotate
History
Anabaena PCC 7120 all1290
Replicon Chromosome Coordinates 1533026 (stop)
lt- 1533628 (start-TTG) Predicted Length
301 amino acids Strand Complementary Function
PvuII DNA methyltransferase Predicted Cyanobacteri
al orthologs none
1533931
Justification IC Inferred by curatorIDA
Inferred by direct assay IEP Inferred from
expression pattern IGI Inferred from genetic
interaction IMP Inferred from mutant
phenotype IPI Inferred from physical
interaction TAS Traceable author statement NAS
Nontraceable author statement ND No biological
data available
IC Inferred by curator
Anab7120
Proteus vulgaris
Salmonella paratyphi
Streptomyces spectabilis
Streptomyces netropsis
Inferred by curator seems to fit the situation.
Click on it.
Neisseria meningitidis
More
20
Main Menu
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Annotate
History
Anabaena PCC 7120 all1290
Replicon Chromosome Coordinates 1533026 (stop)
lt- 1533628 (start-TTG) Human Length 301
amino acids Strand Complementary Function PvuII
DNA methyltransferase Predicted Cyanobacterial
orthologs none
1533931
Comment

Anab7120
Proteus vulgaris
Salmonella paratyphi
Streptomyces spectabilis
Streptomyces netropsis
You would now fill in the reasons leading you to
your assertion regarding the start codon. For
now, just press the Enter key.
Neisseria meningitidis
More
21
Main Menu
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Annotate
History
Anabaena PCC 7120 all1290
Replicon Chromosome Coordinates 1533026 (stop)
lt- 1533931 (start-TTG) Human Length 301
amino acids Strand Complementary Function PvuII
DNA methyltransferase System Cyanobacterial
orthologs none
A
Anab7120
Proteus vulgaris
Salmonella paratyphi
Streptomyces spectabilis
Streptomyces netropsis
The start codon has been changed. In a like
fashion, a name is given to the orf, and
functional annotation added. Now that the
activity seems real, Im inspired to look through
the literature, and... it turns out that Bancroft
et al (1989) described in vivo protection of
Anabaena DNA against PvuII! Press
.
Neisseria meningitidis
Continue
More
Continue
22
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History
Anabaena PCC 7120 all1290
Replicon Chromosome Coordinates 1533026 (stop)
lt- 1533931 (start-TTG) Human Length 301
amino acids Strand Complementary Gene name(s)
avaXM Function Putative type II DNA cytosine
methyltransferase (CAGCTG-specific) Human
Classification Type II beta (N4) Human
Activity Protects against PvuII
Experiment In vivo activity
exists Experiment Cyanobacterial orthologs none
A
A
A
A
A
Anab7120
Proteus vulgaris
Salmonella paratyphi
Thats how the annotation ends up. Now anyone
clicking on Human or Experiment will find the
annotations you made, and anyone mousing over one
of the annotator icons ( ) will get to your
name, and your e-mail address.
Streptomyces spectabilis
A
End
More
23
Scenario 3
Anotation ProcessSummary

• Each assertion is accompanied by a justification
  linked to the annotator
• Interface provides tools for trying out
  different start codons and assessing their
  validity
• Interface provides different alignments for the
  assessment of orf function
• In the end, human expertise is required to
  recognize the critical information

There are no new tools here, just a comprehensive
interface to put these tools at the service of
human annotators who prefer to do science rather
than click keys. The proposal seeks resources to
support the development of this interface.
To quit, right click, then click End Show.