Adventures in HRP

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Adventures in HRP

• Ellyn Daugherty
• San Mateo Biotech Career Pathway www.SMBiotech.com
  
• AEEDaugher_at_aol.com www.BiotechEd.com
• EMC/Paradigm Publishing www.emcp.com/biotech
• VWR/Sargent-Welch www.sargentwelch.com

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San Mateo Biotechnology Career
Pathway www.SMBiotech.com
Giving motivated teenagers and adults the skills
to pursue several career and academic options.
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Partnerships with Industry and Academia
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Biotechnology is an opportunity to teach science
in a way that results in science literacy
research skills career awareness for virtually
every student.

• Students learn the skills and gain the confidence
  and self-directedness that are necessary for
  independent research(science fair projects) and
  higher-level science courses.
• Students see meaning and application in their
  math, biology and chemistry.
• Students learn the science and business of
  biotech so that they are better prepared to make
  decisions about their futures and society.

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The HRP Activities are found in Chapter 6

• Text - Chapter 6 and 14
• Lab Manual - Labs 6f-6h, 14a
• Student CD Tutorials for basic skills
• Course Planner/Instructors Guide

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Biotech 3 HRP labs

• Peroxidase Assay
• HRP extraction from HR Root
• HRP assay using PAGE
• HRP assay w/ TMB indicator
• HRP used in ELISAs

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How Do You Make the Phosphate Buffer?
You need 100 mL of a 25 mM NaH2PO4 H20, pH 7
buffer.
conc. desired X volume desired X molecular
weight amount of solute in mol/L
in L in g/mol
in g
Convert 25 mM 0.025 mM and convert 100 mL
0.1 L
0.025 mol/L X 0.1 L X 138 g/mol
0.345 g NaH2PO4 H20
0.345 g of NaH2PO4 H20 is measured measured on
a balance and put into an appropriate vessel.
Then, solvent is added up to the 100 mL
graduation.
100 mL
Adjust pH to 7 and add dH20 to 100 mL
add solvent (dH20) to 75 mL
0.345 g NaH2PO4 H20
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HRP is 43 kD
50 kD
35 kD
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My Professional Goals
Biotechnology

• Biotechnology programs in every high school, CTE
  center, and community college, tailored to the
  student population and industry and community
  needs
• Aligned and articulated programs in HS and CC so
  that teenagers and adults have the same platform
  from which to enter specialized programs
• To make it as easy as possible for educators to
  do the other 2

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www.BiotechEd.com
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www.BiotechEd.com
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Get help starting or improving a biotech program !
Ellyn Daugherty SM Biotech Career
Pathway www.SMBiotech.com www.BiotechEd.com www.em
cp.com/biotech www.sargentwelch.com/biotech AEEDau
gher_at_aol.com
JEFF GANDY 800.848.9033 jgandy_at_emcp.com
Holly Ahern 512-789-3155 holly_ahern
_at_vwreducation.com
Sandy Duffey for preview copy sduffey_at_emcp.com