Kitajima 1983 Significance of phosphorylation of phosphofructokinase - PowerPoint PPT Presentation

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Kitajima 1983 Significance of phosphorylation of phosphofructokinase

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Title: Kitajima 1983 Significance of phosphorylation of phosphofructokinase


1
Kitajima 1983Significance of phosphorylation of
phosphofructokinase
2
F1. Purity of various PFKs
native PFK

de-P PFK



P-PFK native digest



digest MW
standards
de-P PFK de-P PFK P-PFK P-PFK native native
native PFK de-P PFK P-PFK native
digest digest MW standards
3
Table 1. Activities Pi of various PFKs
4
Table 1. Activities Pi of various PFKs
5
F2a. vo vs.ATP
6
F2a. vo vs. ATP
7
F2b. vo vs. F6P
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Table 2
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F3a. Binding of ATP _at_ 30 µM
10
F3a. Binding of ATP _at_ 30 µM
11
F3b. Binding of F6P _at_25 µM
12
F6. pH vs ATP quenching of fluorescence
13
F7. Activity vs pH
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