MCB 545

1
MCB 545

• abstract template
• a general guide.

2
1.Start with general big pictureVarious yeasts
and other fungi can infect animal and plant cells
alike. These eukaryotes are not sensitive to
most antibiotics and those few compounds that can
kill yeast have limited utility.
3
2.State the specific problemFor example, the
pathogenic yeast Candida albicans often becomes
resistant to the anti-fungal drug XYZ.
Understanding the mechanism(s) by which this
pathogen acquires resistance is critical for
understanding pathogenesis and new strategies for
drug design.
4
What you specifically propose to doSince the
budding yeast S. cerevisiae has been shown to be
a great genetic model system for the C. albicans
drug resistance response, we reasoned that
elucidating the molecular mechanisms by which
drug resistance arises in budding yeast would
inform us on possible ways in which it might
happen in C. albicans.
5
How you specifically plan to do what you
proposeThus, we will perform a genetic screen
for budding yeast mutants that are resistant to
two different anti-fungal drugs ABC and XYZ.
Since these two drugs have very different
structures and function we expect to uncover more
than one resistance pathway.