Mic 201 Lecture

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Mic 201 Lecture 13 Control of Microbial Growth
Please be aware that the whole chapter 20 is
assigned for you to read. This lecture is a
summary and does not replace the textbook
reading! We will also watch a movie on Control of
Microbial Growth. If you are not going to attend
the lecture, please get the notes from a
classmate. You will also find a study guide
posted on my web site. I dont expect you to know
the chemical structure of antibacterial
agents. You will find a homework at the end of
this presentation.
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Extended outline (to be used as a study guide as
well)

• Control of microbial growth Inhibition and
  sterilization.
• Bacteriocidal and bacteriostatics.
• Sterilization.
• Decontamination, disinfection and sterilization.
• Microbial load.
• Microbial control in vivo and in vitro.
• Physical antimicrobial growth
• A) Heat
• B) Radiation
• C) Filtration
• Heat sterilization.
• Kinetics of heat sterilization.
• Effect of temperature on viability graphs.

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• Moisten heat vs. dry heat.
• Decimal reduction times.
• Log of D vs time graphs.
• Thermal death times.
• Spores and heat sterilization.
• Autoclaves and autoclaving conditions.
• The nature of the medium and the nature of the
  killing process.
• Pasteurization bulk and flash.
• Radiation sterilization types of radiation
  used.
• Decimal reduction value.
• Sources of ionizing radiation.
• Filter sterilization and type of filters.

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• Chemical antimicrobial control antimicrobial
  agents in vitro.
• Effect of antimicrobial agents on growth Cidal
  and static agents.
• Measuring antimicrobial activity. Minimum
  inhibitory concentration. Tube dilution technique
  and agar diffusion method.
• Antiseptics, disinfectants and sterilants.
• Antimicrobial agents used in vivo.
• Synthetic antimicrobial drugs. Selective
  toxicity.
• Growth factors analogs and sulfa drugs.
• Antibiotics. Antibiotics affecting protein
  synthesis or RNA polymerase.
• Penicillins and Cephalosporins.
• Aminoglycosides, macrolide and tetracyclines.
• Antimicrobial drug resistance.

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Microbial growth control
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Homework to be turned in on 05/25. Please answer
briefly the following questions.

• What is the difference between an antiseptic and
  a disinfectant?
• What disinfectants are used for the sterilization
  of water?
• Distinguish antibiotics from growth factor
  analogs.
• What is a broad-spectrum antibiotic?
• Why does antibiotic resistance occur?