Textile: Enzyme Applications

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Textile Enzyme Applications
Get the Look You Want with Enzymes
Laccase
Cellulase
Amylase
Novozymes Biotech, Inc. is the Leading Industrial
Enzyme Producer in the world.
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The Enzyme, Laccase is a Tool for creating new
looks in Blue Jeans

• See Biotimes Novozymes Biotech, formerly Novo
  Nordisk) pg 10-11
• Laccase (produced through genetic engineering)
  can help to create the Cool Jeans
• Abrasion
• Desizing
• Bleaching
• Conventional bleaching agents brighten every
  thread of denium, whereas this enzyme only goes
  for indigo!
• Unlike most other fabrics, denium is dyed before
  being woven. In fact only the vertical yarn is
  dyed.

3
Blue Jean Experiment Protocol(Dye Transfer
Inhibition) Laccase-catalyzed

• Materials (compliments of Novozymes
  Biotech)
• Reagent 1 Chicago sky blue 6B (textile dye).03
  mM in H2O
• Reagent 2 methyl syringate 0.2 M in ETOH is an
  accelerator
• Reagent 3 laccase (recombinant enzyme 38U/ml
  in H2O
• deionized water
• pipettors, tips, microfuge tubes
• Protocol 1 (Dye Formation) Takes 1
  minute for RXN
• Test 1ml water 20 micro liter 1 1micro
  liter 2 --- ---gtBlue
• Protocol 2 (Dye Bleaching) observe reaction
  for 4 minutes
• Test Add 1micro liter of 3 to tube with blue
  solution -gtpink-gtpale yellow -gt clear

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Dye Transfer Inhibition at work
Has laccase
NO Laccase
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Laccase Enzymes

• Laccases are a family of multicopper oxidases
  that bear a close resemblance, particularly in
  amino acid sequence, to ascorbate oxidase (found
  in cucumbers) and mammalian plasma protein
  ceruloplasmin. First isolated in 1883 from Rhus
  venicifera, the Japanese lacquer tree, laccases
  are also commonly found in fungi such as the
  lignolytic white-rot fungus Trametes versicolor.
  They have also recently been discovered in
  bacteria.
• Their physiological function is still under
  intense investigation although they are
  implicated in the synthesis and/or degradation of
  the biopolymer lignin, wound response mechanisms
  and the morphogenesis of microorganisms.
• They have a broad specificity that enables them
  to catalyse the one electron oxidation of a range
  of substrates. These substrates, usually phenolic
  in nature, provide electrons for the concomitant
  four electron reduction of atmospheric O2 to
  water. Catalysis takes place via the four copper
  centres which are classified according to their
  electronic properties.

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Laccase Enzymes

• Currently the catalytic properties of laccases
  are being exploited for a range of technological
  applications such as the bioremediation of soils
  and water and the development of environmentally
  friendly processes in the pulp and paper
  industry.
• Much work has gone into the sequencing, cloning
  and mutagenesis of laccase genes from
  microrganisms.
• Recently several crystal structures containing
  the full complement of Cu atoms have been
  reported.

Ribbon representation of the X-Ray
crystallographic structure of Trametes versicolor
laccase reported by Piontek and coworkers in
2002.